NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep Kit

• Optimized protocol offers lower PCR bias and fewer off-target reads
• Fast library prep protocol
• Low input – As low as 1 ng of genomic DNA
• Flexible barcode options
• Automation protocols are now available for the Revvity Sciclone® NGSx Workstation to automate your 16S sequencing
• Functionally tested on the Illumina® MiSeq® sequencer

Optimized 16S Protocol Offers Lower PCR Bias and Fewer Off-target Reads

The protocol incorporated in the NEXTFLEX 16S V3 – V4 Amplicon-Seq Kit offers better sequencing results than can be obtained using traditional 16S sequencing protocols. The incorporation of the second PCR step in the protocol for the addition of the sample-specific index reduces the number of off-target reads typically encountered during amplicon sequencing.

Increase 16S V3 – V4 throughput and reduce errors with automation

A NEXTFLEX® automation protocol is now available on the Sciclone NGSx workstations to help labs increase their throughput and reduce human errors. Automated versions of the NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep kits are available by request.

Analysis Powered by Cosmos-Hub®

The NEXTFLEX 16S V3-V4 rRNA Amplicon-seq panels are now available bundled with access to Cosmos-Hub; an online software solution that enables fast and easy analysis of complex microbiome data. Cosmos-Hub gives scientists user-friendly access to version-controlled and validated 16S pipelines. The machine-learning powered software also enables rapid data interpretation through a comparative analysis software with features such as tables, heatmaps, bar charts, multiple Alpha & Beta Diversity indexes, abundance distribution plots, differential abundance testing, as well as comprehensive statistics between groups. Learn more about Cosmos-Hub.

Revvity 16S rRNA Analysis - Online Demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the Cosmos-Hub^® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.