NEXTFLEX Poly(A) Beads 2.0

Why poly(A) selection with oligo(dT) magnetic beads

For intact total RNA, poly(A) selection with oligo(dT) magnetic beads delivers focused mRNA enrichment that standardizes input for RNA-seq. This approach supports short-read and long-read bulk RNA-seq, 3′ mRNA-seq, transcriptome profiling, and differential expression workflows with a fast, automation-ready magnetic protocol. Use poly(A) selection when your study targets polyadenylated mRNA and RNA quality is high; choose rRNA depletion instead for degraded RNA or when interrogating non-polyadenylated transcripts (e.g., lncRNA), ensuring the right upstream cleanup for your downstream library prep.

For background on why enriching poly(A)+ transcripts improves RNA-seq signal, see our blog RNA-seq: a multilayered view of the mRNA lifecycle.

Figure 1: Representative Poly(A) Enrichment Profile. NEXTFLEX Poly(A) Beads 2.0 produce clean poly(A)+ mRNA with low residual rRNA. From 5 µg total RNA MCF-7 cells, ≈ 1.5% of RNA-seq reads mapped to rRNA, indicating effective removal of rRNA background prior to RNA-seq library preparation.

Reducing rRNA reads in RNA-seq libraries

Total RNA typically contains ~ 80–90% rRNA, so sequencing it directly wastes reads and compute. NEXTFLEX Poly(A) Beads 2.0 hybridize to 3′ poly(A) tails, enabling rapid magnetic separation that enriches polyadenylated mRNA and lowers rRNA/background before RNA-seq library preparation. The on-magnet workflow elutes in small volumes with no precipitation step required to preserve yield, support low-input RNA, and standardize mRNA isolation across samples for all compatible RNA-seq kits. The result is cleaner poly(A)+ mRNA and improved transcript detection for transcriptome profiling and differential expression.

Figure 2: NEXTFLEX Poly(A) Beads 2.0 yield lower rRNA carryover across input amounts. Poly(A)+ mRNA was isolated from Universal Human Reference RNA (Agilent) using NEXTFLEX Poly(A) Beads 2.0 and a competitor poly(A) kit at 1000 ng, 100 ng, and 10 ng.

Seamless integration with NEXTFLEX RNA ecosystem

NEXTFLEX Poly(A) Beads 2.0 slot cleanly into Revvity’s broader NEXTFLEX RNA-seq accessory portfolio , letting you tailor each run to sample type and throughput. Other upstream modules include our ribodepletion portfolio . Downstream, you can scale from pilot sets to 384-plex studies with NEXTFLEX RNA-Seq 2.0 UDI Barcodes or use UDI-UMI adapter plates, adding molecular barcodes when duplicate suppression matters. Adapter lots are color-balanced and QC-verified for index purity, helping limit barcode bleed-through in large pools. Explore the full accessory line to build a single-vendor workflow that grows from low-input discovery to high-throughput studies.

Automation-scripts available

Pre-built scripts for Revvity workstations cut hands-on time e while preserving the metrics that matter the most. Ideal for labs balancing speed, reproducibility, and walk-away convenience.

Plant transcriptomics

• Decsi, K., Ahmed, M., Rizk, R., Abdul-Hamid, D., Vaszily, Z., & Tóth, Z. (2024). Transcriptome datasets of maize plant cultures treated with humic- and amino acids. Data Brief 57:110900. https://doi.org/10.1016/j.dib.2024.110900 .

Model organisms/fundamental biology

• Higdon, A.L., Won, N.H., & Brar, G.A. (2024). Truncated protein isoforms generate diversity of protein localization and function in yeast. Cell Systems 15(4):388–408.e4. https://doi.org/10.1016/j.cels.2024.03.005.