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    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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  • Products
    • Research & Development
      • Genomic Analysis
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        • Nucleic Acid Isolation
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        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
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      • Protein Analysis
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      • Cell Analysis
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      • Research Solutions
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        • Biologics
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        • Disease Research
        • Target Class
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        • Functional Genomic Screening Solutions
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    • Clinical & Diagnostics
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        • Molecular Cytogenetics
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      • Infectious Diseases
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        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
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        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
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      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
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        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
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      • Allergy
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      • Neurodegeneration
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        • Neurogranin
        • BACE1
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      • Rapid Patient Testing
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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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    • Platforms & Automation
      • Nucleic Acid Purification
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        • chemagic IVD Instruments
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        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

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      • Automated Liquid Handling
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        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

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      • Integrated Lab Automation
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        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
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        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Detection Solutions
        • Plate Reader Accessories
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        • Liquid Scintillation Cocktails
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        New! Cellometer™ Ascend™

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      • Imaging
        • Cellular Imaging & Analysis
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        New! Cellometer™ Ascend™

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

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      • Sample Homogenization
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        • Sample Centrifugation
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        New! Cellometer™ Ascend™

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      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
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        • EUROIMMUN Instruments
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        • Clinical Applications
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      • Consumables & Accessories
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        New! Cellometer™ Ascend™

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      • Instrument Service & Maintenance
        • On Demand Equipment Service
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        New! Cellometer™ Ascend™

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      New! Cellometer™ Ascend™

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    • Consumables & Accessories
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    • Signals Software
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      • All Solutions
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
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      • Functional Genomic Screening Services
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      • Cell Panel Screening
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      • Cell Line Engineering
        • CHOSOURCE CLE Services
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      • Viral Vector Engineering and Manufacture
        Preclinical services

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      Preclinical services

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
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        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
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        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • CHOSOURCE Platform
        • AAV Production Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      Investor Day 2024

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  • Brands
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    • Popular Revvity Product Brands
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    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

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  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

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    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

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Library Prep Kits

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NEXTFLEX NGS library prep kits for Illumina and Element Biosciences sequencing platforms

NGS library preparation is a critical process that involves converting raw DNA or RNA samples into a format suitable for sequencing on high-throughput platforms. This preparation includes fragmenting the nucleic acids, repairing ends, adding sequencing adapters, and enriching target regions—all essential steps to ensure accurate and comprehensive sequencing data. Our NEXTFLEX® kits simplify these steps with verified, high-quality protocols and strong technical support, making the library preparation process more efficient and reliable. Furthermore, our kits are designed for full compatibility with leading sequencing platforms such as Illumina® and Element Biosciences™, ensuring they integrate smoothly into your existing laboratory setups and workflows.

We offer a comprehensive portfolio of NEXTFLEX® Next-Generation Sequencing (NGS) library preparation kits designed to streamline your genomic workflows and deliver exceptional results. Recognizing the demands of modern research, we've ensured that our products enable seamless integration with automated liquid handling systems. This focus on automation allows for increased throughput, reduced hands-on time, and enhanced reproducibility, empowering researchers to accelerate their discoveries while maintaining robustness from the very beginning of their workflows.

We provide a diverse range of library prep kits tailored to various applications in genomics research. For DNA sequencing, our kits support whole-genome sequencing , targeted sequencing, and amplicon sequencing, facilitating the exploration of genetic variations and structural genomic changes. In transcriptomics, our RNA-seq kits are designed for mRNA sequencing, total RNA sequencing, and strand-specific RNA sequencing, enabling detailed insights into gene expression and regulation. We also offer specialized kits for small RNA and microRNA sequencing, which are vital for studying gene silencing and post-transcriptional regulation mechanisms. Complementing our core kits, we provide an extensive selection of accessory products —including Universal Blockers, NGS Cleanup Beads, ribodepletion reagents , and barcodes—that are compatible with most other library prep kits on the market including Illumina™, NEB™, Watchmaker™, Twist™, etc. This versatility allows researchers to enhance their existing workflows without the need to alter established protocols, offering cost-effective solutions that maintain flexibility and performance.

  • NGS adapters: Barcoded adapters for all of your NGS multiplexing needs.
  • Small RNA-seq library prep: Gene silencing and post-transcriptional regulation of gene expression can be investigated by small RNA-seq, which allows for the profiling of all of the small RNA and miRNA present in samples.
  • RNA-seq library prep: Profiling for the analysis of the expression level of RNAs in a given cell or population of cells.
  • DNA-seq library prep: Library prep kits for whole genome sequencing.
  • Targeted sequencing: Targeted resequencing for a highly targeted approach that analyses specific genomic regions.
  • 16S rRNA sequencing: 16S amplicon panels used to identify and compare bacteria present within an environmental sample.
  • Single cell DNA-sequencing: Amplification of DNA from single cells and other limited DNA templates for NGS and other applications.
  • Reproductive genetic research: Streamlined PGT-A preparation


For research use only. Not for use in diagnostic procedures.

 

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NEXTFLEX NGS library prep kits for Illumina and Element Biosciences platforms

Explore our solutions

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Ribodepletion Solutions

Revvity offers solutions based on either CRISPR-Cas9 depletion or using a hybridization-probe approach to remove uninformative molecules prior to sequencing and improve your ability to detect biologically relevant transcripts in single cell sequencing, whole blood samples, and even complex communities containing host and bacteria RNA.

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16S rRNA Library Prep Solutions

Revvity now brings flexibility to your microbial community profiling projects with three 16S library prep kits, each covering different regions in the 16S rRNA gene. These kits are available alone or bundled with the CosmosID-Hub® analysis solution, delivering user-friendly access to version-controlled 16S pipelines.

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NGS Barcode Adapters

All of Revvity’s NGS adapters incorporate unique dual indexes (UDI). Revvity now also offers adapters with both unique molecular adapters (UMI) and UDIs. The information below will help you decide what adapter is best for your application.

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WGS library prep kits

Whole Genome Sequencing (WGS) enables researchers to decode the complete DNA sequence of an organism’s genome, providing an exhaustive, base-by-base overview of its genetic material. 

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RNA sequencing library prep kits

RNA sequencing (RNA-seq) is used to analyze the quantity, and the sequence of all RNA transcripts present in a sample at any given time, which is often referred as transcriptome.

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Library Prep OEM

Accelerate your time to market with OEM and custom NGS library prep solutions from Revvity. Collaborate with our experts for flexible, reliable, and innovative products that streamline workflows and deliver reproducible results.

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NEXTFLEX Tech Support

At Revvity we understand that the success of your next-generation sequencing (NGS) projects depends not only on the quality of the reagents you use but also on the support you receive throughout your research journey.

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NEXTFLEX Quality Assurance

Explore Revvity's ISO 9001-certified production of NEXTFLEX® NGS library preparation kits. Our dedicated QA team ensures transparency, traceability, and consistent product performance.

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Featured resources

Video

Extracellular vesicles in liquid biopsies for neurological disorders

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Blog

SPRI technology: principles, bead composition, and bead-to-sample ratio impact

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Blog

Unraveling the power of unique molecular identifiers in cancer research.

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Application note

TotalSeq™ and DepleteX® technologies for efficient analysis of the cellular transcriptome

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Blog

Challenges in analyzing data with UMI-UDIs in next-generation sequencing.

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Flyer
Flyer
A streamlined approach to genotyping with low-pass WGS

This flyer illustrates Revvity's new streamlined arroach to genotyping with low-pass NGS

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Scientific Poster
Scientific Poster
A Universal Day Zero Infectious Disease Testing Strategy Leveraging CRISPR-based Sample Depletion and Metagenomic Sequencing

This poster showcases the benefits of using CRISPRclean technology for zero day infectious disease screening.

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Application Note
Application Note
Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing

The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation, which often includes proper sample/tissue homogenization and nucleic acid extraction. As the lab scales their sample throughput and their need for consistency in complex workflows, many have integrated automation-enabled protocols into their workflows. In this application note, a semi-automated workflow for nucleic acid extraction from liver tissue is presented, which leverages the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic 360 nucleic acid extractor, with optional use of a JANUS automated liquid handling workstation.

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Flyer
Flyer
Considerations when selecting the right barcoded adapters

Considerations when selecting the right barcoded adapters

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Flyer
Flyer
CRISPR depletion enables sensitive identification of viral and bacterial populations in complex samples

This flyer showcases the benefits of using CRISPRclean technology.

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Technical Note
Technical Note
CRISPRclean® depletion technology: A new tool to better understand rare diseases

Enhanced RNA-Seq delivers double the genes detected with confidence

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Application Note
Application Note
CRISPRclean® Plus for SARS-CoV-2 Shotgun Metatranscriptomic Sequencing

CRISPR-based ribodepletion removes RNA “noise” from assays, allowing key insights into variants, as well as co-infections and host responses.

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Datasheet
Datasheet
CRISPRclean™ Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria)

CRISPR-based ribodepletion strategy optimized with stranded RNA prep increases coverage of lower-expressing transcripts from complex biological samples.

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Datasheet
Datasheet
CRISPRclean™ Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat)

CRISPR-based ribodepletion strategy improves sensitivity for the detection of lower expressing transcripts from mammalian tissues

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Brochure
Brochure
Decoding library complexity

This document provides guidance on the applications in which NEXTFLEX UDI-UMI Barcodes can be used.

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Technical Note
Technical Note
DepleteX™ Globin Depletion Kit

Remove contaminating globin fragments for RNA-Seq

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Technical Note
Technical Note
DepleteX™ Single Cell RNA Boost Kit

Remove abundant and uninformative fragments prior to scRNA-Seq

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Application Note
Application Note
DOPlify v2 with PicoPLEX App Note

In this appplication note the DOPlify® v2 WGA kit and Takara® PicoPLEX® Single Cell WGA kit were compared and performed comparably when analyzed for whole chromosome copy number variations and targeted panel coverage when using 5-cell samples as input.

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Application Note
Application Note
DOPlify WGA kit App Note

The DOPlify® kit from Revvity is a whole genome amplification kit designed and optimized specifically to amplify the genome from single cells and limited DNA templates.

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Flyer
Flyer
Efficient DNA purification using SPRI technology

This flyer describes showcases the NEXTFLEX NGS Cleanup Bead specifications and uses cases.

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Infographic
Infographic
Elevate your single-cell sequencing: Beat the stats

Discover simple strategies to overcome single cell sequencing obstacles in our infographic.

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Flyer
Flyer
End-to-end metagenomics solutions.

This flyer showcases Revvity's metagenomic portfolio as well as Cosmos-Hub award winning analysis solution.

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Flyer
Flyer
End-to-end whole genome sequencing metagenomics solutions

This flyer describes Revvity and Cosmos-Hubs solution for WGS for metagenomics.

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Literature - Publication Review
Literature - Publication Review
Extracellular vesicles: Discovery tools to pioneer the next wave of biomarkers

Extracellular vesicles (EVs) represent a fascinating frontier in biomarker discovery. Secreted by virtually all cell types, these vesicles circulate in biological fluids carrying molecular cargo that reflects their parent cells’ composition - offering unique insights into cellular function and disease processes through minimally invasive sampling. Effective isolation and characterization methods are required to make the most of the valuable information contained within EVs. Traditional approaches are often labor-intensive and demand large sample volumes, but recent advances help address these limitations. This literature review explores the emerging role of EVs as biomarkers and the technical advances overcoming key barriers to their application in research and diagnostic applications. Learn about: Evolving EV isolation and analysis technologies using minimal sample volumes High-throughput miRNA profiling using acoustic trapping combined with NEXTFLEX™ Small RNA-Seq Multiplex detection of EV surface proteins using TotalSeqTM-A antibody-based immuno-PCR For research use only. Not for use in diagnostic procedures.

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Product Info
Product Info
FASTQ file containing control data for the DepleteX Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing control data for the DepleteX™ Single Cell RNA Boost Kit.

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Product Information Bulletin
Product Information Bulletin
FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit.

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Flyer
Flyer
Gel-free small RNA library prep kit for Illumina sequencing

This flyer describes the benefits of the NEXTFLEX® Small RNA-Seq Kit v4 kit

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Flyer
Flyer
Get the answers you need with single-cell sequencing

Flyer showcasing Revvity's solutions for key steps of your single-cell sequencing journey.

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Application Note
Application Note
High-throughput, end-to-end Cell Free DNA (cfDNA) analysis workflow from plasma

Benefit of an automated cfDNA analysis workflow The presence of double stranded, circulating cell free DNA (cfDNA) in blood plasma was discovered more than 70 years ago. Soon after, a link between cfDNA and leukemia as well as autoimmune disease was found. Recently, the development of cfDNA-based prenatal genetic testing and the realization that cfDNA can be used to detect and monitor tumor specific mutations in cancer patients has increased the interest in cfDNA analyses. Read the application note to review the results and how much time you could save by automating your cfDNA analysis workflow using Revvity’s automated solution including blood fractionation, cfDNA isolation, library prep, QC, and quantitation.

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Technical Note
Technical Note
Identify more genes per UMI with CRISPRclean® and Spatial Gene Expression

Boost informative transcripts and detect low expression genes in fresh frozen tissues

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