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Maternal Cell Contamination Test -MCC
Maternal Cell Contamination Test -MCC
Test code:
N4060
Maternal Cell Contamination Test -MCC
Maternal Cell Contamination (MCC) - Presence of maternal cells or DNA in fetal sample/DNA is known as MCC
| Test Code | N4060 |
|---|---|
| Test Summary | Maternal Cell Contamination (MCC) - Presence of maternal cells or DNA in fetal sample/DNA is known as MCC |
| Turn Around Time | 7 days |
| Acceptable Sample Types | Amniotic Fluid , Chorionic Villi , Cord Blood , Cord Tissue , Cultured Cells - AF , Cultured Cells - CVS , DNA, Isolated , Fetal Blood , Maternal Blood , Products of Conception , Whole Blood (EDTA) |
| NY Approved | No |
*TAT starts after the sample and all required sample information is received at the processing laboratory.
**The CPT codes listed are in accordance with Current Procedural Terminology, a publication of the American Medical Association, and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party.
This testing service has not been cleared or approved by the U.S. Food and Drug Administration. Testing services may not be licensed in accordance with the laws in all countries. The availability of specific test offerings is dependent upon laboratory location.
**The CPT codes listed are in accordance with Current Procedural Terminology, a publication of the American Medical Association, and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party.
This testing service has not been cleared or approved by the U.S. Food and Drug Administration. Testing services may not be licensed in accordance with the laws in all countries. The availability of specific test offerings is dependent upon laboratory location.
Test code:
N4060
This test is not available in your region United States, please select your nearest laboratory to request more information.
Revvity Sites Globally
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Test information
Test description
Maternal Cell Contamination (MCC)Presence of maternal cells or DNA in fetal sample/DNA is known as Maternal Cell Contamination (MCC). Highly polymorphic STR (short tandem repeat) loci are used to differentiate between fetal and maternal samples/DNA, identify extent of Maternal Cell Contamination if present and thus avoid erroneous reporting. At present, the test can confidently identify 10% Maternal Cell Contamination or greater.
Indications for testing
MCC
One of the risks associated with prenatal /fetal testing is maternal cell contamination (MCC), which occurs when fetal specimen is mixed with maternal blood or tissue. The risk of MCC is associated with any fetal sample such as chorionic villus, POCs, Cord Blood, Amniotic Fluid, etc. If MCC is present, the maternal DNA may interfere with the fetal genetic testing thereby resulting in inaccurate reporting and diagnosis. This test will enable us to identify if a specific fetal sample has MCC. In order to perform this test a maternal blood sample is required along with the fetal sample.
Condition description
The presence of both maternal and nonmaternal alleles for each fetal marker indicates the fetal specimen is not contaminated. MCC is confirmed when both alleles in the fetus are maternal.
Test methods and limitations
MCC - Testing includes amplification of highly polymorphic short tandem repeat (STR) markers with fluorescently labelled primers; fragment sizing of the resulting PCR products using a genetic analyzer.
Detailed sample requirements
DNA, Isolated
| Collection |
Required DNA Quantity by Test Type*:
|
|---|---|
| Sample Condition |
* Required DNA Quality: High molecular weight DNA (>12kb). A260/A280 reading should be ≥ 1.8. A260/230 a ratio range of 1.8 to 2.2. Contact the laboratory for specific amounts if total ng cannot be met. |
| Shipping |
Ship overnight at ambient temperature. |
| SPECIAL SAMPLE INSTRUCTIONS |
|
Products of Conception
| Collection Container(s) |
Sterile, screw-top container filled with tissue culture transport medium. |
|---|---|
| Collection |
All prenatal specimens will be tested for maternal cell contamination (MCC). Send maternal blood (EDTA tube) for comparison. Transport products of conception and/or fresh tissue (minimum: 10 mg) in a sterile, screw-top container filled with tissue transport medium. Do not place in formalin. If the specimen is too large for a normal collection tube, a larger sterile container can be used. |
| Sample Condition |
If the autopsy is performed: Facia lata, diaphragm, tendon, skin, tissue from internal organs (if fresh), chest wall cartilage (particularly if macerated), or placenta from fetal side. If no autopsy is performed: Placenta from fetal side is preferred, e.g., villi. Umbilical cord is also acceptable. State clearly in the TRF whether the tissue was of fetal or placental in origin. |
| Shipping |
Shipped immediately at ambient temperature or with a cold pack, especially under hot weather to the laboratory by overnight express with arrival Monday-Friday only. Alternatively, fresh POC and/or tissue samples can be snap-frozen and kept at -80°C. Frozen tissue samples should be shipped overnight on dry ice with arrival Monday-Friday only - NO WEEKEND SHIPMENTS! |
| SPECIAL SAMPLE INSTRUCTIONS |
For prenatal samples, Revvity Omics will provide a shipping label to use for shipping the sample to our lab. Please call 1 (866) 354-2910 to arrange this. At this time, you will also be connected to a laboratory genetic counselor to answer any questions about the testing. For samples originating outside of the United States, please contact your nearest Revvity Omics laboratory for more information. |
Chorionic Villi
| Collection Container(s) |
EDTA (Purple Top) or ACD (Yellow Top) |
|---|---|
| SPECIAL SAMPLE INSTRUCTIONS |
Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types. |
Cultured Cells - AF
| SPECIAL SAMPLE INSTRUCTIONS |
Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types. |
|---|
Cultured Cells - CVS
| SPECIAL SAMPLE INSTRUCTIONS |
Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types. |
|---|
Whole Blood (EDTA)
| Collection Container(s) |
EDTA (purple top) |
|---|---|
| Collection |
Infants (< 2-years): 2 to 3 mL; Children (>2-years): 3 to 5 mL; Older children and adults: Minimum 5mL. The blood tube should be inverted several times immediately after blood collection to prevent coagulation. |
| Sample Condition |
Store at ambient temperature. Do not refrigerate or freeze. |
| Shipping |
Ship overnight at ambient temperature ensuring receipt within 5-days of collection. |
| SPECIAL SAMPLE INSTRUCTIONS |
Clotted or hemolyzed samples are not accepted. |
Amniotic Fluid
| SPECIAL SAMPLE INSTRUCTIONS |
Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types. |
|---|
Cord Blood
| Collection Container(s) |
EDTA (Purple Top) or ACD (Yellow Top) |
|---|---|
| Collection |
Collect 1 to 3 mL of fetal blood. |
| Sample Condition |
Store at ambient temperature. Do not refrigerate or freeze. |
| Shipping |
Ship overnight at ambient temperature ensuring receipt within 5 days of collection. |
Cord Tissue
| SPECIAL SAMPLE INSTRUCTIONS |
Please contact a Revvity Omics Laboratory genetic counselor for information on prenatal test requests and acceptable sample types. |
|---|
How To Order
Step 1
Choose Your Test
Select the correct test for your patient, and download and fill out the Clinical Genomics test requisition form.
Step 2
Collect Sample
Obtain a sample for testing from the patient using one of the provided Revvity Omics test packs.
Step 3
Send Samples
Send samples and all required forms back to Revvity for processing using pre-paid shipping label.
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