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  • Home
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  • Protein Analysis
  • Immunoassays
  • Alpha Assays
  • AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points

AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points

Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
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AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
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Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Alpha Terbium SureFire Ultra Multiplex
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
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The Alpha SureFire® Ultra™ Multiplex phospho-Insulin Receptor β (Tyr1150/1151) + Total Insulin Receptor β assay kit is used to measure both the phosphorylation (on Tyr1150/1151) and total levels of endogenous Insulin Receptor β in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Insulin-1 Receptor β phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

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Feature Specification
Application Cell Signaling
Sample Volume 10 µL
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The Alpha SureFire® Ultra™ Multiplex phospho-Insulin Receptor β (Tyr1150/1151) + Total Insulin Receptor β assay kit is used to measure both the phosphorylation (on Tyr1150/1151) and total levels of endogenous Insulin Receptor β in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Insulin-1 Receptor β phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

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Product variants
Unit Size: 500 assay points
Part #:
MPSU-PTINR-K500
List price
USD 4,307.46
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Unit Size: 10,000 assay points
Part #:
MPSU-PTINR-K10K
List price
USD 26,010.00
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Unit Size: 50,000 assay points
Part #:
MPSU-PTINR-K50K
List price
USD 80,760.36
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Unit Size: 100 assay points
Part #:
MPSU-PTINR-K-HV
List price
USD 1,125.00
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Video Play Button
Video Play Button
AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Video Play Button
Video Play Button
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Alpha Terbium SureFire Ultra Multiplex
AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Alpha Terbium SureFire Ultra Multiplex Image
AlphaLISA SureFire Ultra Multiplex Detection Kit
Video Play Button
Video Play Button
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Product information

  • Overview
  • Specifications
  • Video gallery

Overview

The Alpha SureFire Ultra Multiplex p-Insulin Receptor β + Total Insulin Receptor β assay kit is used to measure both phosphorylated and total levels of endogenous Insulin Receptor β in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Insulin Receptor β analysis, and the 545 nm (Tb) signal corresponds to the total Insulin Receptor β analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra Multiplex
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
Insulin Rec. β
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Metabolic
Unit Size
50,000 assay points

Video gallery

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AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points
Video Play Button
Video Play Button
AlphaLISA SureFire Ultra Multiplex Human Phospho-IR-β (Insulin Receptor Beta) (Thr1150/1151 & Total) Detection Kit, 50,000 Assay Points
Video Play Button
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Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Guide
AlphaLISA SureFire Ultra: the ultimate guide for successful experiments

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay

Several biological processes are regulated by...

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Guide
AlphaPlex quick start guide

The AlphaPlex™ reagent system was designed to enable fast and easy transition from well-established AlphaLISA™ assays to...

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