Search all

Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

Cellaca PLX High-throughput Image Cytometer

Knowledge base

The Cellaca™ PLX is a high-throughput image cytometer for cell analysis workflows, delivering fast cell counts (up to 24 samples in <5 mins), concentration, and viability readouts. Additionally, the Cellaca PLX can detect surface markers, apoptotic markers, and transduction and transfection efficiency – all at the lab bench.

For research use only. Not for use in diagnostic procedures.

Part #: PLX-SYS1

Imaging Modality:

Fluorescence, Brightfield

Request more information Request a quote

Cellaca PLX High-throughput Image Cytometer

Cellaca-PLX-with-reagents

Cellaca PLX High-throughput Image Cytometer

Request a quote Request more information

View related products

Product variant

Fluorescence, Brightfield

Imaging Modality: Fluorescence, Brightfield

Part #: PLX-SYS1

Request information

Video Player

00:00

Use Up/Down Arrow keys to increase or decrease volume.

Product information

Overview

The Cellaca PLX image cytometer provides versatile cell analysis ranging from simple cell counts, concentration, and viability readouts, to more complex multiplexed assays, all using only 15-50 µL of sample. The sensitivity of the system allows for the analysis of up to 24 samples using dual fluorescence and brightfield imaging in under 5 minutes, and single sample multiplexing with four channels in 1 minute.

Multiplex panels for immunophenotyping, apoptosis, and fluorescent protein expression shorten the time to downstream processing with Cellaca PLX low fluorescence slides, dedicated reagents, built-in assays, and configurable data reports. The Cellaca PLX features user friendly software, automation compatibility, and is 21 CFR Part 11 ready. Expert support is provided to integrate the system into current workflows to streamline cell line development, cell isolation, gene delivery, and various research applications.

Key features and benefits of the Cellaca PLX:

Elevated throughput - run up to 24 samples in under 5 minutes using dual fluorescence and brightfield imaging to count both isolated nuclei and cells
Multiplexing capabilities - shorten time to downstream processing (one minute per sample with four channels) using our custom reagents kits for surface marker, apoptosis, and fluorescent protein analysis
Small sample volume - as little as 15 µL of cell sample needed
Intuitive workflows - including a favorites feature for saving customized experiments and commonly used assays with automatic data analysis and reporting
Automation ready - robotic integration ability with via API
21 CFR Part 11 ready - add-on that includes audit trail, user access control, and digital signature

Additional product information

Cellaca PLX applications

With the Cellaca PLX platform, you can:

Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
Perform routine viability measurements as well as apoptosis functional assays to determine cell health.

Cellaca PLX optimized assay reagents and kits

The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.

Available assay kits include:

Immune cell phenotyping
Apoptosis detection
High-throughput cell viability
Fluorescent protein analysis

Low auto-fluorescent consumables

Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.

Software that makes multiplexing easy

Stress-free Cellaca PLX software comes with preloaded, step-by-step templates and methodologies to streamline multiplex analysis, providing a benchtop solution for quick, reliable measurements of cell counts and concentrations, surface marker staining, viability, and apoptosis. And you get customizable results for presentations, too.

A Cellaca instrument for every analysis

The Cellaca instrument line comprises the Cellaca MX high-throughput cell counter, with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system, with brightfield and five fluorescent channels, for multichannel analysis.

	Cellaca PLX	Cellaca MX (MX-AOPI)
Channels	Brightfield, Blue, Green, Red, Far Red	Brightfield, Green, Red
Number of fluorescent channels	13 (6 per scan)	3 (2 per scan)
Excitation LED	370 nm, 475 nm, 531 nm and 628 nm	470 nm, 527nm
Emissions Filters	452 nm, 534 nm, 605 nm, 655 nm and 692 nm	534 nm, 655 nm
Commonly Used Compatible Dyes	Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, KIRAVIA Blue 520™*	Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7
Counting Speed	Fluorescence 4-channel scan in 1 minute (per plate)	Trypan Blue - 2 seconds Fluorescence 2-channel scan- Less than 8 seconds (per well)
Volume (per well)	15 µL in slides 50 µL - 200 µL in counting plates	50 µL - 200 µL in counting plates
Size/Diameter Range	5 µm - 80 µm	5 µm - 80 µm
Concentration Range	1x10⁵ - 1x10⁷ cell/mL	1x10⁵ - 1x10⁷ cell/mL
IQ/OQ Option	Yes	Yes
21 CFR Part 11 Ready Option	Yes	Yes
Automation Compatible	Yes	Yes
Dimensions	13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm)	13 in x 13 in x 16 in (33 cm x 33 cm x 41 cm)
Weight	42 lbs (19 kg)	42 lbs (19 kg)

Specifications

Physical specifications

Dimensions	13.0 in (W) x 16.0 in (D) x 13.0 in (H)
Weight	20.0 kg

Other specifications

21CFR Compatible	Yes
Automation Compatible	Yes
Brand	Revvity
Imaging Modality	Fluorescence, Brightfield
Model	Cellaca PLX High-Throughput Image Cytometer
Technology	Matrix Software
Unit Size	1 unit

Image gallery

Video gallery

Citations

FAQs

How much volume of my sample do I need to perform a count?

The minimum loading volume of stained sample in the counting plate is 50 µl. Typically, you will stain your cell sample 1:1 with AOPI dual-fluorescent viability dye, so you can perform a cell count with as little as 25 µl of cell sample. For cell-based assays such as immunophenotyping or GFP/RFP, instead of the counting plate, you will use low-fluorescent background slides that only require 15 µl of sample. If you have a low-volume sample, you can perform a cell count in the slides as well.
Can I save the plate for later if I only have a few samples to count?

Yes, load the counting plate with as many or as few samples as you want to count. The Cellaca software will allow you to indicate which wells contain samples you wish to count and the rest of the plate can be saved for later use.
Are the counting plates compatible with multi-channel pipettes?

Yes, the counting plates are compatible with multi-channel pipettes, making it fast and easy to stain and prep your samples. The Cellaca is also compatible with liquid handlers and automation platforms and has an optional automation license add-on, making it easy to scale up your throughput.
What daily start-up or shut-down cycles do I need to run?

There are no daily start-up or shut-down cycles for the Cellaca because there are no internal fluidics and nothing that needs to get cleaned or could clog. The Cellaca uses LEDs as a light source. The only prep you need to do is turn on the instrument and start up the software, and the Cellaca is ready to count your samples right away.
How does the Cellaca count challenging or messy cell types?

The Cellaca is designed to identify live and dead nucleated cells, even in very messy samples with RBCs and debris or in irregular-shaped cell populations. By using the AOPI dual-fluorescence viability dye, only nucleated cells are counted and RBCs and debris are ignored (this method is so specific, it even works in whole blood without lysing!). The Cellaca will show you the images it captured and which cells were counted so that you can visually verify the results and trust your data.