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NEXTFLEX Universal Blockers
NEXTFLEX Universal Blockers
NEXTFLEX NGS Library Prep
| Feature | Specification |
|---|---|
| Automation Compatible | No |
| Product Group | Targeted Sequencing |
Product variants
Unit Size: 8 rxns
Part #:
NOVA-5143231
List price
USD 113.41
Your online price:
Unit Size: 48 rxns
Part #:
NOVA-5143232
List price
USD 237.54
Your online price:
Unit Size: 96 rxns
Part #:
NOVA-5143233
List price
USD 373.42
Your online price:
For research use only. Not for use in diagnostic procedures.
NEXTFLEX Universal Blockers
NEXTFLEX NGS Library Prep
NEXTFLEX Universal Blockers
Product information
Overview
Simplify and reduce the cost of hybridization-based target capture with the NEXTFLEX universal blockers for NGS
- Effective in blocking without being affected by index length or the presence of a unique molecular index (UMI)
- Compatible with all NEXTFLEX barcodes and all other ligation and tagmentation based workflows for Illumina and Element sequencing platforms
- Simplifies and reduces the cost of target capture without sacrificing performance.
Additional product information
Improve the number of on-target reads
How adapter-blocking oligos increase on-target reads
During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule. The NEXTFLEX Universal Blockers are designed to prevent this concatemerization of library molecules. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.
One set of blockers for all applications
The NEXTFLEX Universal Blockers are designed for use with indexed adapters for Illumina sequencing platforms. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI).
Figure 1. NEXTFLEX Universal Blockers (UB) work from 0-19 bp unblocked regions (barcodes and/or UMIs). Competitor A1- and A-3 are two separate products from Competitor A.
Figure 2. NEXTFLEX Universal Blockers can block Illumina TruSeq™ or Nextera™ -style libraries. Competitor A1- and A-2 are two separate products from Competitor A.
The NEXTFLEX Universal Blockers are compatible with ligation-based workflows, such as NEXTFLEX or Illumina TruSeq library prep kits, and with any libraries prepared with NEXTFLEX Dual-Index Barcodes or NEXTFLEX Unique Dual Index Barcodes.
They are also compatible with tagmentation-based workflows, such as Illumina Nextera library prep kits.
Specifications
| Automation Compatible |
No
|
|---|---|
| Product Group |
Targeted Sequencing
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Unit Size |
48 rxns
|
Citations
- Tian, Yijun, et al. "Identify Regulatory eQTLs by Multiome Sequencing in Prostate Single Cells." bioRxiv, 21 June 2024, doi:10.1101/2024.06.19.599704. https://pmc.ncbi.nlm.nih.gov/articles/PMC11213234/
FAQs
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What are universal blockers?
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Do universal blockers work with 6 bp and 10 bp dual indexes?
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Can I use universal blockers with Twist or IDT libraries?
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What concentration should I use the NEXTFLEX Universal blockers at?
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How should I store the universal blockers?
Resources
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Flyer
Simply & reduce the cost of hybridization-based target capture with universal blockers
This flyer describes the benefits of the NEXTFLEX® Universal Blockers
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