NEXTFLEX RNA-seq 2.0 UDI barcodes (8NT index, 1-24)

• UDI adapters for multiplexing up to 384 RNA-seq libraries for Illumina® and Element® sequencing platforms
• Every lot is tested for index purity and functionality by sequencing
• Compatible with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 and the CRISPRclean® Plus Stranded Total RNA Prep with rRNA Depletion
• Compatible with 'T-A' overhang (Illumina’s TruSeq style) ligation-based RNA-seq workflows

The NEXTFLEX® RNA-seq 2.0 UDI barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0 and the CRISPRclean® Plus Stranded Total RNA Prep with rRNA Depletion.

Improve RNA-seq data integrity

The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is tested for index purity and functionality by sequencing.