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  • Who we serve
    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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    • Pharma / Biotech
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  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
        • Single-Cell Sequencing
        • Labeled Nucleotides
        • qPCR
        • Library Prep Kits
        • Magnetic Beads
        • Viral Vector Products
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      • Protein Analysis
        • Binding Assays
        • Immunoassays
        • Microfluidic Protein Characterization
        • Microplate Readers
        • Microplates
        • Recombinant Proteins
        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
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      • Cell Analysis
        • Cell Isolation
        • Cell Lines & Stem Cells
        • Cell Counting and Image Cytometry
        • Cell Health & Viability
        • Cellular Imaging & Analysis
        • Immunoassays
        • In Vivo Imaging
        • Microplates
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      • Research Solutions
        • Biomarker Discovery
        • Cell and Gene Therapy
        • GMP Workflows
        • Immunoassays Solutions
        • pHSense Reagents
        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Microphysiological Systems
        • Biobanking Workflows
        • Agrigenomics Workflows
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    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Revvity Genomics Software
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
        New IVD Mimix™ reference standards for oncology workflows.

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      • Allergy
        New IVD Mimix™ reference standards for oncology workflows.

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      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

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      • Rapid Patient Testing
        New IVD Mimix™ reference standards for oncology workflows.

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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
      • BioLegend
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      • Cellular Imaging Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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      • GPCR Research Reagents
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      • Immunoassays
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      • In Vivo Imaging Reagents
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      • Liquid Scintillation Cocktails
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      • NGS Library Prep Kits
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      • pHSense Reagents
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      • qPCR
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      • Radiochemicals
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      • RNA Interference
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      • Primary and Secondary Antibodies
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    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Automated Liquid Handling
        • Flow Cytometry Cocktail Prep Automation
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Sample Homogenization
        • Homogenizers
        • Homogenizer Accessories
        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Consumables & Accessories
        • Cassettes
        • Cell Harvesters
        • In Vivo Imaging Accessories
        • High-Content Imaging Accessories
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
        • Transfer Membranes
        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Instrument Service & Maintenance
        • On Demand Equipment Service
        • Equipment Service Plans
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      New! Cellometer™ Ascend™

      Explore our cell counting and image cytometry solutions.

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    • Consumables & Accessories
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    • Signals Software
      • All Products
        • Signals One
        • ChemDraw
        • Spotfire®
        • Signals Clinical
        • Signals Notebook
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      • All Solutions
        • Biology
        • Discovery Chemistry
        • Clinical & Translational
        • Specialty Chemicals
        • Food/Flavor/Fragrance
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    • Revvity Omics Services
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Panel Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • AAV Production Platform
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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  • Company
    • Purpose
      • Approach
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      • Our Story
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    • Careers
      • Careers Home
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      • Why Revvity
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    • Investor Relations
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        Investor Day 2024

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        Investor Day 2024

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      • Stock Info
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

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      Investor Day 2024

      Driving meaningful innovation that profoundly impacts science and human lives.

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    • ESG
      • Environmental
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    • News
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  • Resources
    • Product Support
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  • Brands
      Revvity Brands
    • BioLegend

    • EUROIMMUN

    • Revvity Omics

    • Revvity Signals

    • Tulip Diagnostics

    • ViaCord

    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

    • View all Revvity brands
  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

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    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

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NEXTFLEX HT Agrigenomics Low-pass WGS Kit

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NEXTFLEX NGS Library Prep
NEXTFLEX HT Agrigenomics Low-pass WGS Kit
NEXTFLEX NGS Library Prep
NEXTFLEX NGS Library Prep
NEXTFLEX NGS Library Prep

The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA for Low pass whole genome sequencing WGS or Skim-seq applications, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO.

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

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Feature Specification
Automation Compatible Yes
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The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA for Low pass whole genome sequencing WGS or Skim-seq applications, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO.

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

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Product variants
Barcodes: 1 - 24
Unit Size
24 rxns
Part #:
NOVA-5141-70
List price
USD 293.76
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384 rxns
Part #:
NOVA-5141-71
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USD 4,700.16
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Barcodes: 385 - 768
Unit Size
384 rxns
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Barcodes: 769 - 1152
Unit Size
384 rxns
Part #:
NOVA-5141-73
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Barcodes: 1153 - 1536
Unit Size
384 rxns
Part #:
NOVA-5141-74
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USD 4,700.16
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For research use only. Not for use in diagnostic procedures.
Total list price:
USD 293.76
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NEXTFLEX NGS Library Prep
NEXTFLEX HT Agrigenomics Low-pass WGS Kit
NEXTFLEX NGS Library Prep
NEXTFLEX NGS Library Prep
NEXTFLEX HT Agrigenomics Low-pass WGS Kit
NEXTFLEX NGS Library Prep
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Product information

  • Overview
  • Additional product information
  • Specifications

Overview

The NEXTFLEX HT Agrigenomics Low Pass Sequencing Kit is a new, streamlined workflow compatible with Illumina and Element Biosciences platforms. With a wide input range, it is suitable for all kind of plant and animal samples and can be run either manually or in automated workstation. Performance of the libraries has been tested with samples homogenized and extracted with Revvity solutions.

  • Robust – fast and easy protocol
  • Wide input range – from 1 ng to 500 ng
  • PCR-free compatible workflow
  • High throughput – up to 1,536 UDI barcodes available

Additional product information

Low-pass whole genome sequencing (LP-WGS) followed by imputation is becoming the preferred method for genotyping among plant and animal breeders. LP-WGS can capture a much broader spectrum of genetic variation compared to SNP arrays and is not limited by a fixed content, speeding up the selection process and improving the reliability of predictions.  The high computational demands associated with processing and imputing LP-WGS data make it less accessible for many research programs and breeding initiatives.

Revvity and CURIO now deliver a comprehensive solution that includes reagents, automation, and analysis for your WGS data, tackling many of the main challenges encountered by breeders.

Once your sequencing data is received, CURIO generates results in minutes with just a single click. From initial research to large‐scale routine workflows, CURIO provides leading imputation analysis for both plant and animal breeders.

You can find more detailed information here on the Curio website.

Imputation horizontal


Figure 1: Example of imputation analysis detail performed by CURIO on a domestic pig sample (Sus scrofa)

Flexible multiplexing options: Up to 1,536 UDI adapters

Barcodes are supplied with the NEXTFLEX HT Agrigenomics Low Pass Sequencing Kit. We offer 1,536 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs.

Automation compatibility

The NEXTFLEX HT Agrigenomics Low-Pass WGS Kit. is compatible with multiple liquid handlers, including the Fontus™ NGS, Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ™, and Zephyr® G3 NGS workstations.

Workflow

Automation compatibility workflow

 

Online demonstration

You can now schedule a one-on-one 30-minute online demonstration with our experts to cover everything you need to know about the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit protocol and data analysis with Curio. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

A streamlined approach to genotyping with Low-Pass WGS webinar

Watch our on-demand webinar to discover how low-pass whole genome sequencing (LP-WGS) is revolutionizing genotyping in plant and animal breeding. Learn from experts Dr. Charlie Johnson and Dr. Marcel Brun from Texas A&M, and Dr. Pedro Echave from Revvity, along with Shawn Quinn from Curio Genomics.

Agrigenomics webinar

Specifications

Application
DNA-seq library prep
Automation Compatible
Yes
Barcodes
1 - 24
Shipping Conditions
Dual Temperature
Unit Size
24 rxns

Resources

Are you looking for resources, click on the resource type to explore further.

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  • Application Note (1)
  • Flyer (1)
  • Other (1)
1-3 of 3 Resources
Flyer Icon
Flyer
A streamlined approach to genotyping with low-pass WGS

This flyer illustrates Revvity's new streamlined arroach to genotyping with low-pass NGS

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Application Note Icon
Application Note
Modernizing molecular breeding with low-pass WGS

The combination of the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit with the CURIO™ platform offers a fast and robust solution for...

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Other Icon
Other
NEXTFLEX UDI Barcodes Sequences 1536 Set

This excel file includes the sequences of the indexes for the 1536 NEXTFLEX UDI Barcodes.

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SDS, COAs, manuals and more

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Safety data sheet
  • Language
    English
    Country
    United States
    Name
    SDS IconMSDS NEXTFLEX HT Agrigenomics Low-Pass WGS Kit

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IFUs, manuals & more
  • Resource Type
    Manual
    Language
    English
    Country
    -
    Name
    SDS IconNEXTFLEX HT Agrigenomics Low-Pass WGS Kit Manual

Request an Request a Manual/IFU or Contact us for support regarding any other missing documentation like Barcodes, DOC, etc.

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