AlphaLISA SureFire Ultra Multiplex Human Phospho-Rb (Thr821/826 & Total) Detection Kit, 500 Assay Points

The Alpha SureFire® Ultra™ Multiplex p-Rb (Thr821/826) (Eu) + Total Rb ssay kit is used to measure both phosphorylated and total levels of endogenous Rb in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Rb analysis, and the 545 nm (Tb) signal corresponds to the total Rb analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

• No-wash steps, no separation steps
• Multiplexing assay
• Sensitive detection
• No interference from exogenous antibodies
• Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.