The DepleteX® Single Cell RNA Boost Kit employs a CRISPR-based method to selectively remove unwanted reads allowing you to cut through the noise with minimal impact on your workflow and maximum confidence in your results.
Enhance usable single-cell RNA-seq data using DepleteX ® Single Cell RNA Boost Kit
DepleteX ® efficiently removes abundant and uninformative fragments before sequencing, reallocating 50% of sequencing clusters to informative reads that would typically be filtered out during secondary analysis. This kit utilizes Cas9 depletion with an optimized guide set to target ribosomal and mitochondrial mRNA, non-transcriptomic reads, and highly expressed non-variable genes. Additionally, the kit provides a separate tube for non-variable gene depletion, which can be included or excluded as required.
The DepleteX® Single Cell RNA Boost Kit empowers you to reduce noise and optimize usable data in your single-cell RNA sequencing.
Key highlights:
Figure 1: Double the reads mapped to the transcriptome with the DepleteX® Single Cell RNA Boost Kit.
Figure 2: 1.5x improvement in detection with depletion
Shipping Conditions |
Shipped in Dry Ice
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Unit Size |
24 rxns
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Remove abundant and uninformative fragments prior to scRNA-Seq
Please fill out the form below to access a FASTQ file containing control data for the DepleteX™ Single Cell RNA Boost Kit.
Please fill out the form below to access a FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit.
Boost informative transcripts and detect low expression genes in fresh frozen tissues
Combining PacBio® MAS-Seq and Jumpcode Single Cell RNA Boost Kit improves isoform detection sensitivity
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