Skip to main content

DepleteX Single Cell RNA Boost Kit

The DepleteX® Single Cell RNA Boost Kit employs a CRISPR-based method to selectively remove unwanted reads allowing you to cut through the noise with minimal impact on your workflow and maximum confidence in your results.

For research use only. Not for use in diagnostic procedures.
Part number: NOVA-512850
List price: USD 6,000.00
Your price:
USD 6,000.00
USD 6,000.00 /each

Overview

Enhance usable single-cell RNA-seq data using DepleteX ® Single Cell RNA Boost Kit

DepleteX ® efficiently removes abundant and uninformative fragments before sequencing, reallocating 50% of sequencing clusters to informative reads that would typically be filtered out during secondary analysis. This kit utilizes Cas9 depletion with an optimized guide set to target ribosomal and mitochondrial mRNA, non-transcriptomic reads, and highly expressed non-variable genes. Additionally, the kit provides a separate tube for non-variable gene depletion, which can be included or excluded as required.

The DepleteX® Single Cell RNA Boost Kit empowers you to reduce noise and optimize usable data in your single-cell RNA sequencing.

Key highlights:

  • Enhance secondary analysis with ~50% more useful reads
  • Verified for both short-read and long-read sequencing.
  • Efficiently removes ribosomal, mitochondrial, non-transcriptomic reads, and optionally non-variable genes from single-cell libraries
  • Streamlined 3-step protocol
  • Tested with 10X genomics and other single cell sequencing workflows

Additional product information

DepleteX Single Cell RNA Boost Kit reads-mapped-increase v3


Figure 1: Double the reads mapped to the transcriptome with the DepleteX® Single Cell RNA Boost Kit.

Fig 2. DepleteX Single Cell RNA Boost KitS


Figure 2: 1.5x improvement in detection with depletion

Specifications

Shipping Conditions
Shipped in Dry Ice
Unit Size
24 rxns

Selected Publications / Citations / References

  1. Currenti, J., Qiao, L., Pai, R., Gupta, S., Khyriem, C., Wise, K., Sun, X., Armstrong, J., Crane, J., Pathak, S., Yang, B., George, J., Plummer, J., Martelotto, L., & Sharma, A. (2022). STOmics-GenX: CRISPR-based approach to improve cell identity-specific gene detection from spatially resolved transcriptomics. bioRxiv. Advance online publication.
  2. Pandey, A. C., Bezney, J., DeAscanis, D., Kirsch, E., Ahmed, F., Crinklaw, A., Choudhary, K. S., Mandala, T., Deason, J., Hamdi, J., Siddique, A., Ranganathan, S., Ordoukhanian, P., Brown, K., Armstrong, J., Head, S., & Topol, E. J. (2022). A CRISPR/Cas9-based enhancement of high-throughput single-cell transcriptomics. bioRxiv. Advance online publication.

Resources

1-5 of 5 Resources
Technical Note
DepleteX™ Single Cell RNA Boost Kit

Remove abundant and uninformative fragments prior to scRNA-Seq

Product Information Bulletin
FASTQ file containing control data for the DepleteX Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing control data for the DepleteX™ Single Cell RNA Boost Kit.

Product Information Bulletin
FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit

Please fill out the form below to access a FASTQ file containing depletion data for the DepleteX™ Single Cell RNA Boost Kit.

Technical Note
Identify more genes per UMI with CRISPRclean® and Spatial Gene Expression

Boost informative transcripts and detect low expression genes in fresh frozen tissues

Technical Note
Increase scRNA-seq coverage of low-expression genes and isoforms with depletion

Combining PacBio® MAS-Seq and Jumpcode Single Cell RNA Boost Kit improves isoform detection sensitivity

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

Scroll Icon