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  • Immunoassays
  • DELFIA
  • DELFIA Lysis Buffer, 30 mL

DELFIA Lysis Buffer, 30 mL

DELFIA Bottle 100mL
DELFIA Lysis Buffer, 30 mL
DELFIA Bottle 100mL
DELFIA Bottle 100mL
DELFIA Bottle 100mL

DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.

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DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.

View product information
Product variant
Unit Size: 30 mL
Part #:
4005-0010
List price
USD 376.72
Your online price:
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA Bottle 100mL
DELFIA Lysis Buffer, 30 mL
DELFIA Bottle 100mL
DELFIA Bottle 100mL
DELFIA Lysis Buffer, 30 mL
DELFIA Bottle 100mL
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Product information

  • Overview
  • Specifications
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Overview

DELFIA® time-resolved fluorescence (TRF) cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Specifications

Automation Compatible
Yes
Brand
DELFIA
Detection Modality
DELFIA
Product Group
Buffer
Shipping Conditions
Shipped in Blue Ice
Technology
TRF
Unit Size
30 mL

Citations

Resources

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1-2 of 2 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Learn more
Guide Icon
Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

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