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CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion
CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion
Jumpcode-box-image_700x700
| Feature | Specification |
|---|---|
| Automation Compatible | Yes |
| Product Group | Ribodepletion |
Product variant
Unit Size: 24 rxns
Part #:
NOVA-5229960
List price
USD 3,852.54
Your online price:
For research use only. Not for use in diagnostic procedures.
CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion
Jumpcode-box-image_700x700
CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion
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Product information
Overview
- Ideal for analysis of samples containing complex mixtures of eukaryotic and bacterial rRNA
- 3 to 7-fold increase in coverage of SARS-CoV-2 and other genomes in nasopharyngeal (NSP) samples
- Single workflow to detect viral genomic data, microbiome composition, co-infections, and host gene expression
- Streamlined Jumpcode™ CRISPRclean workflow: stranded total RNA library prep with ribodepletion
- Automated on the Sciclone™ G3 NGSx iQ™ liquid handler
Improve your discovery of high-value and biologically interesting transcripts
Powered by CRISPR-Cas9, ribodepletion is highly efficient and programmed to cut ribosomal RNA from human sequences (5S, 5.8S, 18S, 28S, 45S (precursor), mitochondrial 12S and 16S sequences), and 212 bacterial species from all phyla (5S, 16S, and 23S rRNA sequences).
Fully Automated Protocol
A tested method is available to automate Jumpcode Genomics’ CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit on the Sciclone™ G3 NGSx iQ™ liquid handler.
Multiplexing options
The NEXTFLEX™ RNA-seq 2.0 Unique Dual Index Barcodes (1-384) are provided at a concentration optimized for use with Jumpcode Genomics’ CRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria) kit.
Additional product information
| Assay Time | 9 hours |
|---|---|
| Application | Infectious disease surveillance |
| Hands-on Time | 3.5 hours |
| Sample Types | Complex samples with mixture of eukaryotes and prokaryotes including nasopharyngeal, saliva, gut, skin, and fecal |
| Input Quantity | 5 - 100 ng total RNA |
| Depletion Technology | CRISPR-Cas9 mediated |
| Strand Compatibility | >98% directional and strand specific |
| Compatible Species | Human, mouse, rat, and 212 bacterial species |
| Designed to deplete | Human 5S, 5.8S, 18S, and 28S, 45S rRNA precursor, mitochondrial 12S and 16S rRNA genes, 212 bacterial species representing all phyla: 5S, 16S, and 23S rRNA genes |
| Multiplex | Up to 1536 UDI adapter barcodes |
For the US patent, the patent number is US 10,604,802 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/22/2a/ef/6657ca336c9c08/US10604802.pdf
For the EP patent, the patent number is EP3102722 entitled Genome Fractioning. The patent publication is available at https://patentimages.storage.googleapis.com/a7/54/96/52ed494ab92016/EP3102722B1.pdf
Specifications
| Automation Compatible |
Yes
|
|---|---|
| Product Group |
Ribodepletion
|
| Shipping Conditions |
Dual Temperature
|
| Unit Size |
24 rxns
|
References
- Brown, K., Chan, A. P., Siddique, A., Desplat, Y., Choi, Y., Ranganathan, S., Choudhary, K. S., Diaz, J., Bezney, J., DeAscanis, D., George, Z., Wong, S., Selleck, W., Bowers, J., Zismann, V., Reining, L., Highlander, S., Hakak, Y., & Armstrong, J. R. (2022). A Universal Day Zero Infectious Disease Testing Strategy Leveraging CRISPR-based Sample Depletion and Metagenomic Sequencing. Open Forum Infectious Diseases, 9(Supplement_2), ofac492.5801.
- Cerón, S., Clemons, N. C., von Bredow, B., & Yang, S. (2023). Application of CRISPR-Based Human and Bacterial Ribosomal RNA Depletion for SARS-CoV-2 Shotgun Metagenomic Sequencing. American Journal of Clinical Pathology, 159(2), 111–1151. doi.org/10.1093/ajcp/aqac135
- Uranga C, Nelson KE, Edlund A and Baker JL (2022) Tetramic Acids Mutanocyclin and Reutericyclin A, Produced by Streptococcus mutans Strain B04Sm5 Modulate the Ecology of an in vitro Oral Biofilm. Front. Oral. Health 2:796140. doi.org/10.3389/froh.2021.796140
Resources
Are you looking for resources, click on the resource type to explore further.
Flyer
CRISPR depletion enables sensitive identification of viral and bacterial populations in complex samples
This flyer showcases the benefits of using CRISPRclean technology.
Application Note
CRISPRclean® Plus for SARS-CoV-2 Shotgun Metatranscriptomic Sequencing
CRISPR-based ribodepletion removes RNA “noise” from assays, allowing key insights into variants, as well as co-infections and host...
Datasheet
CRISPRclean™ Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria)
CRISPR-based ribodepletion strategy optimized with stranded RNA prep increases coverage of lower-expressing transcripts from...
Application Note
Increase bacterial species detection in microbiome stool samples
Performance comparison study of CRISPRclean® Plus and Illumina® Ribo-Zero™ Plus for microbiome analysis
Flyer
NEXTFLEX RNA-seq portfolio
This flyer illustrates the breadth of the NEXTFLEX RNA-seq Portfolio
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