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  • Home
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  • Research & Development
  • Protein Analysis
  • Immunoassays
  • Alpha Assays
  • AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points

AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points

AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
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AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
Video Play Button
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
Video Play Button

The AlphaLISA™ immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Feature Specification
Application Protein Quantification
Dynamic Range 1.1 - 1,000 ng/mL
Limit of Detection 1.1 ng/mL  
Limit of Quantification 3.4 ng/mL  
Sample Volume 5 µL
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The AlphaLISA™ immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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Product variants
Unit Size: 100 assay points
Part #:
AL3027HV
List price
USD 701.22
Your online price:
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Unit Size: 500 assay points
Part #:
AL3027C
List price
USD 2,323.68
Your online price:
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Unit Size: 5,000 assay points
Part #:
AL3027F
List price
USD 15,347.00
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 2,323.68
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AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
Video Play Button
AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
Video Play Button
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead
AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead image
IgG (mouse) AlphaLISA Detection Kit
Video Play Button

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Product information

  • Overview
  • Specifications
  • Video gallery

Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Heme oxygenases are enzymes that are involved in the degradation of heme. They cleave the heme ring to create billiverdin, which then becomes bilirubin due to the action of bilirubin reductase. This cascade allows for the removal of heme in damaged red blood cells as well as heme bound irreversibly to molecules such as carbon monoxide. Heme oxygenases have been characterized as markers for oxidative stress, metabolic disorders and inflammation. Heme oxygenase 2 is present mainly in the brain. Its role appears to be protective against oxidative stress. Production of carbon monoxide from the cleavage of the heme ring seems to play a protective role against malaria by poisoning the hemoglobin essential for the parasite growth.

Specifications

Application
Protein Quantification
Automation Compatible
Yes
Brand
AlphaLISA
Detection Modality
Alpha
Dynamic Range
1.1 - 1,000 ng/mL
Limit of Detection
1.1 ng/mL  
Limit of Quantification
3.4 ng/mL  
Product Group
Kit
Sample Volume
5 µL
Shipping Conditions
Shipped in Blue Ice
Target
HO-2
Target Class
Biomarkers
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Unit Size
500 assay points

Video gallery

AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points
AlphaLISA Human Heme oxygenase-2 Detection Kit, 500 Assay Points

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