| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human IRF5 Aggregate Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K cells/mL and incubated overnight in 10% FBS containing medium. Cells were harvested, adjusted to 5 x 106 cells/mL and treated with 100 nM Calyculin A for 3 hours. Following treatment, cells were washed with HBSS + 0.1% BSA and lysed with Lysis Buffer at a final concentration of 10 x 106 cells/mL.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRF5
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
1 vial
|
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