Skip to main content
Menu
Revvity logo
Contact us
  • Request a quote
  • Contact sales
  • Contact customer care
  • Application support
  • Instrument service request
  • Training request
US
Search all
  • Who we serve
    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

      Visit BioLegend.com

    • Pharma / Biotech
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Clinical Laboratories
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Healthcare Professionals
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Contract Research Organizations
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
        • Single-Cell Sequencing
        • Labeled Nucleotides
        • qPCR
        • Library Prep Kits
        • Magnetic Beads
        • Viral Vector Products
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Protein Analysis
        • Binding Assays
        • Immunoassays
        • Microfluidic Protein Characterization
        • Microplate Readers
        • Microplates
        • Recombinant Proteins
        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Cell Analysis
        • Cell Isolation
        • Cell Lines & Stem Cells
        • Cell Counting and Image Cytometry
        • Cell Health & Viability
        • Cellular Imaging & Analysis
        • Immunoassays
        • In Vivo Imaging
        • Microplates
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Research Solutions
        • Immunoassays Solutions
        • pHSense Reagents
        • Biomarker Discovery
        • Cell and Gene Therapy
        • GMP Workflows
        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Physiological Model Solutions
        • Biobanking Workflows
        • Agrigenomics Workflows
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

        Learn more

      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Endocrinology
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Allergy
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Rapid Patient Testing
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      New IVD Mimix™ reference standards for oncology workflows.

      Learn more

    • Reagents
      • BioLegend
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Cellular Imaging Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • chemagic Nucleic Acid Purification Kits
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • CRISPR Technologies
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • GPCR Research Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Immunoassays
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • In Vivo Imaging Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Liquid Scintillation Cocktails
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • NGS Library Prep Kits
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • pHSense Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • qPCR
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Radiochemicals
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • RNA Interference
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Primary and Secondary Antibodies
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Automated Liquid Handling
        • Flow Cytometry Cocktail Prep Automation
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Sample Homogenization
        • Homogenizers
        • Homogenizer Accessories
        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Consumables & Accessories
        • Cassettes
        • Cell Harvesters
        • In Vivo Imaging Accessories
        • High-Content Imaging Accessories
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
        • Transfer Membranes
        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Instrument Service & Maintenance
        • On Demand Equipment Service
        • Equipment Service Plans
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      New! Cellometer™ Ascend™

      Explore our cell counting and image cytometry solutions.

      Learn more

    • Consumables & Accessories
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Signals Software
      • All Products
        • Signals One
        • ChemDraw
        • Spotfire®
        • Signals Clinical
        • Signals Notebook
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • All Solutions
        • Biology
        • Discovery Chemistry
        • Clinical & Translational
        • Specialty Chemicals
        • Food/Flavor/Fragrance
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Revvity Omics Services
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Cell Panel Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

      Learn more

    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Gene Expression Systems
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

      Learn more

    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

    Learn more

  • Company
    • Purpose
      • Approach
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Our Story
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Leadership
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Careers
      • Careers Home
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Why Revvity
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Search Jobs
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Investor Relations
      • Events
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      • Financials
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      • Stock Info
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      Investor Day 2024

      Driving meaningful innovation that profoundly impacts science and human lives.

      Learn more

    • ESG
      • Environmental
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Social
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Governance
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • News
      • Announcements
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Awards
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Media Alerts
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Press Coverage
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Resources
    • Product Support
      • Application Support Knowledge base (ASK)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SDS Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • COA/TDS Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Manual/IFU Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SpectraViewer
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • RAD Calculator
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Resource Center
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Blog
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Events
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Customer Training
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Help Center
      • Order Support
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Contact Us
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Technical Support
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Instruments Support & Service
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SDS Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • COA/TDS Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Manual/IFU Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Training Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Cell Line Terms & Conditions
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • FAQs
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Software Downloads
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Knowledge Base
      • Application support knowledge base (ASK)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Sample homogenization applications and protocols
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Newborn screening disorders
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • TB testing services
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Investigator led studies
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Tuberculosis (TB)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    Tech documents, at your fingertips.

    Quickly find and download manuals, safety documents, certificates of analysis and more.

    Search now

  • Brands
      Revvity Brands
    • BioLegend

    • EUROIMMUN

    • Revvity Omics

    • Revvity Signals

    • Tulip Diagnostics

    • ViaCord

    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

    • View all Revvity brands
  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

    View our story

    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

Shop
Request a Quote
Contact Us
US
Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

australia.webp Australia
austria.webp Austria
belgium.webp Belgium
brazil.webp Brazil *
canada.webp Canada
china.webp China *
denmark.webp Denmark
finland.webp Finland
france.webp France
germany.webp Germany
hong-kong.webp Hong Kong *
india.webp India *
ireland.webp Ireland
italy.webp Italy
japan.webp Japan *
luxembourg.webp Luxembourg
mexico.webp Mexico *
netherlands.webp Netherlands
norway.webp Norway
philippines.webp Philippines *
republic of korea.webp Republic of Korea *
singapore.webp Singapore *
spain.webp Spain
sweden.webp Sweden
switzerland.webp Switzerland
thailand.webp Thailand *
uk.webp United Kingdom
usa.webp United States
Login/Register here
Revvity web shop online account

Get exclusive pricing on all online purchases.

Login to your Revvity.com account for your account's pricing, easy re-ordering from favorites & order history, priority order processing, and dynamic order tracking.

Login Register
Revvity Omics portal accounts

Initiate a new order or access test status and results for clinical genomics or newborn screening services.

View login options

Power your prep: 10% OFF sample prep instruments, accessories, and consumables! Terms and conditions apply. Learn more.

Breadcrumb
...
  • Home
  • Knowledge Base
  • CellCountingSub Section
  • Surface Marker and Intracellular Protein Detection
Cell Counting and Image Cytometry

Surface Marker and Intracellular Protein Detection

Section
Cellometer Applications
Celigo Applications
Cell Counting Method Selection
Cell Counting and Image Cytometry FAQs
Cell-based Assays for Bioprocessing
Cell-based Assays for Gene Therapy Development
Cellometer Applications
Modern Virology Assays
Sub Section
Fluorescent Assays
Automated Analysis of Primary Cells
Brightfield Cell Analysis
Cell Analysis for Breweries and Biofuels
Cell Concentration and Viability for Blood-based Samples
Cell Counting with a Hemocytometer
Cell Viability for Clean and Messy Samples
Fluorescent Assays
Immuno-oncology Research Using Cellometer Instruments
Miscellaneous Cell Types
Topic
Surface Marker and Intracellular Protein Detection
Apoptosis
Autophagy Detection
Cell Cycle Analysis
Expression Analysis
Surface Marker and Intracellular Protein Detection

Introduction to surface and intracellular proteins markers

Surface and intracellular proteins are markers that are important for identifying specific type of cells and characterizing cell populations. Traditionally, these assays are performed on flow cytometers. More recently,  image-based cytometry method has been utilized for many standard surface and intracellular protein detection assays to provide a simpler and more rapid method for cell population analysis. The Cellometer Vision CBA* image cytometer performs surface marker and intracellular protein fluorescence analysis to characterize cell populations. Below are examples of assays that have been performed using the Cellometer image cytometry method.

*This section refers to the Cellometer Vision CBA image cytometer which has been superseded by the Cellometer Spectrum system.

Human Activated T Cell – CD44-APC

Human T cells were stained with APC conjugated anti-CD44 and imaged on the Cellometer Vision CBA. The counted brightfield image shows T cells automatically identified by the Cellometer software and outlined in green color. The fluorescent APC image shows red surface marker staining of positively stained T cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that more than 95% of the cells were positive for CD44.
 

human activated T cell CD44 APC
Bright Field Counted Image

Brightfield counted image

Fluorescence Image

Fluorescence image

Human Activated T Cell – CD44-PE

Similar to the experiment above, Human T cells were stained with phycoerythrin (PE) conjugated anti-CD44 and imaged on the Cellometer Vision CBA. The counted brightfield image shows T cells automatically identified by the Cellometer software and outlined in green color. The fluorescent PE image shows orange surface marker staining of positively stained T cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that more than 95% of the cells were positive for CD44.

Human Activated T Cell – CD44-PE
Human Activated T Cell – CD44-PE BF

Brightfield counted image

Human Activated T Cell – CD44-PE FI

Fluorescence iImage

Macrophage in mouse bone marrow – CD11b-PE

Primary bone marrow sample was stained with PE conjugated anti-CD11b, a cell surface receptor found on the surface of several cell types including mononuclear phagocytes a.k.a macrophages. Twenty microliters of the stained sample were imaged on the Cellometer Vision CBA system. The green circles shown in the brightfield image represent automatically counted and outlined mononuclear cells. The fluorescent image shows bright staining profile of cells that are positive for CD11b. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that about 84% of the cells are positive for CD11b.

Macrophage in Mouse Bone Marrow – CD11b-PE
Macrophage in Mouse Bone Marrow – CD11b-PE BF

Brightfield counted image

Macrophage in Mouse Bone Marrow – CD11b-PE FI

Fluorescence image

Human jurkat cells – CD3-PE

Cultured Jurkat cells were stained with PE conjugated anti-CD3 surface marker and imaged on the Cellometer Vision CBA. The counted brightfield image shows Jurkat cells that were automatically identified by the Cellometer software and outlined in green color. The fluorescent PE image shows orange surface marker staining of CD3 positive Jurkat cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that more than 80% of the cells were positive for CD3.

Human Jurkat Cells – CD3-PE
Human Jurkat Cells – CD3-PE BF

Brightfield counted image

Human Jurkat Cells – CD3-PE FI

Fluorescence image

Human PBMC – CD3-FITC

Primary peripheral blood mononuclear cell (PBMC) sample was stained with FITC conjugated anti-CD3 surface marker and imaged on the Cellometer Vision CBA. The counted brightfield image shows cells that were automatically identified by the Cellometer software and outlined in green color. The fluorescent FITC image shows green surface marker staining of CD3 positive mononuclear cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that nearly 90% of the cells were positive for CD3.

Human PBMC – CD3-FITC
Human PBMC – CD3-FITC BF

Brightfield counted image

Human PBMC – CD3-FITC FI

Fluorescence image

Human PBMCs – CD3-PECy5

Similar to the CD3-FITC experiment above, a primary peripheral blood mononuclear cell (PBMC) sample was stained with anti-CD3 PE-Cy5 conjugated tandem dye and imaged on the Cellometer Vision CBA. Note that this tandem dye is a combination of phycoerythrin (PE) and cyanine (Cy5), has a broad emission spectra and was imaged using the APC filter module. The counted brightfield image shows cells that were automatically identified by the Cellometer software and outlined in green color. The fluorescent image shows a reddish surface marker staining of positively stained mononuclear cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that about 65% of the cells were positive for CD3.

Human PBMCs – CD3-PECy5
Human PBMCs – CD3-PECy5 BF

Brightfield counted image

Human PBMCs – CD3-PECy5 FI

Fluorescence image

Human PBMCs – CD19-PECy5

A primary peripheral blood mononuclear cell (PBMC) sample was stained with anti-CD19 PE-Cy5 conjugated tandem dye and imaged on the Cellometer Vision CBA. Note that this tandem dye is a combination of phycoerythrin (PE) and cyanine (Cy5), has a broad emission spectrum and was imaged using the APC filter module. The counted brightfield image shows cells that were automatically identified by the Cellometer software and outlined in green color. The fluorescent image shows a reddish surface marker staining of positively stained B-cells. The mean fluorescent intensity values for each cell were exported to flow cytometry software FCS Express™ and plotted as a histogram. The gated data shows that about 10% of the cells were positive for CD19 B-cells.

Human PBMCs – CD19-PECy5
Human PBMCs – CD19-PECy5 BF

Brightfield counted image

Human PBMCs – CD19-PECy5 FI

Fluorescence image

Mouse cells – Mouse IgG-FITC and RFP

Mouse Cells – Mouse IgG-FITC and RFP
Mouse IgG-FITC BF

Brightfield counted image

Mouse IgG-FITC FI-1

Fluorescence image

Mouse IgG-FITC FI-2

Fluorescence image

CD44 – PE

CD44 – PE data
CD44 – PE BF

Brightfield counted image

CD44 – FL

Fluorescence image

Human Lymphocytes – CD4-PE

Human lymphocytes – CD4-PE

Mouse Thymocytes – CD8-PE and CD4-APC

Mouse thymocytes – CD8-PE and CD4-APC

Mouse Splenocytes – CD8-PE and CD4-APC

Mouse splenocytes – CD8-PE and CD4-APC

MHC Dextramer – CD8-FITC and Dextramer-PE

MHC Dextramer – CD8-FITC 1
MHC Dextramer – CD8-FITC 2
MHC Dextramer Dextramer-PE

Human T cells and cytokines – CD8-PE and INFg-FITC

Human T Cells and Cytokines – CD8-PE and INFg-FITC

Intracellular proteins for protein pathway research

Various complex proteins exist in cells that are generated or removed by cellular functions. These proteins can be used as an indicator to measure certain protein pathways or functions of the cells to determine if they are operating normally or abnormally. These intracellular proteins can be fluorescently labeled and measured by the Cellometer Vision CBA image cytometer to determine the cell population associated with these proteins.

Heat shock protein in MNNG cancer cells – HSP90-PE and GFP

Heat Shock Protein in MNNG Cancer Cells – HSP90-PE and GFP
Heat Shock Protein in MNNG Cancer Cells BF

Brightfield counted image

Heat Shock Protein in MNNG Cancer Cells FL-1

Fluorescence image

Heat Shock Protein in MNNG Cancer Cells FL-2

Fluorescence image

Heat shock protein in MNNG cancer cells – Grp78-Alexa fluor 594 and GFP

Heat Shock Protein in MNNG Cancer Cells – Grp78-Alexa Fluor 594 and GFP
Heat Shock Protein in MNNG Cancer Cells BF-2

Brightfield counted image

Heat Shock Protein in MNNG Cancer Cells FL-1-2

Fluorescence image

Heat Shock Protein in MNNG Cancer Cells FL-2-2

Fluorescence image

Stem cell analysis for regenerative medicine

Stem cell markers are often used to determine the stem cell population in blood, cord blood, bone marrow, and adipose tissue. These cells are important for research in regenerative medicine, and the ability to quickly characterize a stem cell population in a cell sample can greatly improve research efficiency. The Cellometer Vision CBA image cytometer can be utilized to measure cell population of some of the common stem cell markers.

Mouse iPS cells – SSEA-1-PE

Mouse iPS Cells – SSEA-1-PE
Mouse iPS Cells – SSEA-1-PE BF

Brightfield counted image

Mouse iPS Cells – SSEA-1-PE FL

Fluorescence image

Human iPS cells – SSEA-4-PE

Human iPS Cells – SSEA-4-PE
Human iPS Cells – SSEA-4-PE BF

Brightfield counted image

Human iPS Cells – SSEA-4-PE FL

Fluorescence image

Human iPS cells – TRA-181-PE

Human iPS Cells – TRA-181-PE
Human iPS Cells – TRA-181-PE BF

Brightfield counted image

Human iPS Cells – TRA-181-PE FL

Fluorescence image

CACO2 cancer stem cells – CD133-FITC

CACO2 cancer stem cells – CD133-FITC
CACO2 cancer stem cells – CD133-FITC BF

Brightfield counted image

CACO2 cancer stem cells – CD133-FITC FL

Fluorescence image


For research use only. Not for use in diagnostic procedures.

ON THIS PAGE
  • Introduction to surface and intracellular proteins markers
  • Intracellular proteins for protein pathway research
  • Stem cell analysis for regenerative medicine
Revvity Logo
  • Company
    • Purpose
    • Approach
    • Our Story
    • Leadership
    • Careers
    • News
    • Investor Relations
    • ESG
  • Connect
    • Contact Us
    • Offices & Authorized Distributors
    • Newsletter
    • BioLegend
    • EUROIMMUN
    • Revvity Signals
    • Tulip Diagnostics
    • ViaCord
  • Policies
    • All Policies
    • Privacy Policy
    • Terms & Conditions
    • Cookie Policy
    • Accessibility
  • Support
    • Order Support
    • Technical Support
    • Customer Care
    • Request Service Visit
    • Customer Training
    • Sales
    • Contact Us By Phone
    • FAQs
  • Facebook
    FB
  • Instagram
    IG
  • LinkedIn
    LI
  • Twitter
    X
  • Youtube
    YT

©2025 Revvity - All rights reserved

Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.