Design and assembly of modular pin-point base editors for precise correction of pathogenic SNVs

In this scientific poster, we present our pipeline for the design and development of bespoke modular Pin-point™ base editors with defined editing activities across therapeutic DNA target sequence space.

Read how we have established an arrayed screening platform and optimised gRNA libraries to characterise the editing activities of diverse combinations of Cas and deaminase modules at scale.

Knowledge obtained from the arrayed screens is used to guide the design of parallel screens to determine the efficiency and specificity of favourable base editor assemblies across pooled libraries covering all targetable pathogenic SNVs, with promising candidates validated and further optimised in cellular models harbouring SNV target sequences.

The Pin-point™ base editing platform technology is available for clinical or diagnostic study and commercialization under a commercial license from Revvity.