Omni Bead Ruptor Elite Bead Mill Homogenizer Accessories

Ocular tissues, ranging from small, soft perfused tissues to larger, tougher collagenous ones, are commonly selected for analysis during preclinical pharmacokinetic studies for ophthalmic drugs. The process of tissue homogenization and subsequent extraction needs to be optimized for proper downstream drug detection from these various matrices. In this study, bead mill homogenizers were selected for use for its efficiency and effectiveness in preparing complete homogenates. This methodology allows for the extraction of Cyclosporine from eight different ocular tissues – cornea, conjunctiva, iris-ciliary body (ICB), sclera, eyelid, lens, retina-choroid, and lacrimal gland. Due to the different phospholipid profiles between the tissues, however, varying amounts of matrix suppression were encountered during method development; to reduce the effect on analyte response, a phospholipid removal plate was used. The developed LC-MS/MS assay is robust, as well as demonstrates analytical reproducibility and efficiency for the quantification of Cyclosporine in the assayed ocular tissues. Acceptable selectivity, matrix factor, and dilution integrity were demonstrated in detail. Similarly, the methodology was flexible enough to allow for reanalysis of over-range samples as well as in posterior tissues with expected lower Cyclosporine levels. For research use only. Not for use in diagnostic procedures.

Stool metagenomics workflow using optimized vs non-optimized bead matrices for bead mill homogenizer for NGS library prep.

Bead mill homogenizers enable rapid and efficient lysis of biological materials, including plants via high-impact forces generated from beads in an enclosed tube to dissociate samples. We explore the efficiency of vertical intra-tube bead milling motions of 7mL and 30mL tube and spiral grinding motions of a 50mL tube using the Omni Bead Ruptor Elite unit. Results indicate protein extraction yields were consistent run-to-run, in different tube sizes, and that full homogenization was achieved in less than a minute for all cases using the same homogenization settings within a given sample type. Thus, having one unit to support varying sample tube sizes allows bead milling to be leveraged for rapid and efficient sample homogenization across various sample input sizes/volumes for protein applications.

biomarker discovery and precision med research workflow flyer

Bead mill sample homogenization, exemplified by the Omni Bead Ruptor Elite™ homogenizer, is a rapid and efficient method for sample disruption. However, for longer processing, the kinetic energy generated can increase sample temperature to potentially affect heat-sensitive analytes. We evaluated the passive cooling effect of the 7 mL carriage on larger volume samples, with the carriage and buffers pre-cooled to various temperatures. The results showed that using a carriage equilibrated at -80 °C in combination with chilled buffers effectively kept processing temperatures below 15 °C for up to 1 minute of processing time for larger volume samples though for samples that are in 2mL tubes, active chamber cooling is preferred utilizing a cryo cooling unit.

The comminution step in sample prep is critical for the effectiveness of the QuEChERS method, a type of dSPE, for the multiple pesticide extraction and detection. We evaluate the robustness of bead milling in 50 mL tubes for rapid homogenization of various types of certified organic produce, such as strawberries, soybeans, and apples spiked with 17 component pesticide standards at 100 ppb. Full homogenization by visual inspection was achieved in 30 seconds for all assayed samples of varying density with implemented settings. Pesticides were then extracted and analyzed by GC-MS for % recovery.

The ability to detect pathogens throughout the food supply chain is an important component of food safety research. Just as the adoption of high-throughput methods such as PCR has expedited molecular detection of microorganisms vs more traditional time-consuming and labor-intensive methods, bead milling allows for an efficient and rapid sample prep method in foods, such as spinach. In this application note, both the bead mill and rotor-stator homogenizers were shown to fully process spinach samples spiked with recombinant E. coli while maintaining their viability. Both methods delivered a 76% cell recovery and a PCR detection limit of 1x10-6 CFUs/mL. Thus, the protocol and methods outlined may help expedite pathogen detection without necessitating a longer pre-enrichment step.

Within agricultural and food studies, genomic analysis plays a large role in elucidating insights into genome modifications, breeding studies, pesticide resistance studies, and identification of plant species. Technologies and methods that can help labs optimize their workflows are valuable, especially if they lend themselves to the throughput, scale, and consistency in nucleic acid extractions for downstream assays, such as PCR or NGS. In this application note, bead mill homogenizers are identified as a robust solution for sample preparation of plant tissues such as spinach leaves, offering an efficient alternative for DNA and RNA extraction compared to time-consuming methods like enzymatic digestion, with resulting homogenates being suitable for downstream analysis. For research use only. Not for use in diagnostic procedures.

Plant biology research helps our understanding behind complex mechanisms of plant growth, development, and response to environmental conditions. It touches various aspects from molecular biology, genetics, and ecology through applicable areas in agricultural production, GM crop research, and natural extracts for pharmaceuticals or cosmetics. Within sample preparation for genetic or genotypic analysis, raw sample homogenization is a critical first step to nucleic acid extraction. Bead milling facilitates the disruption and homogenization of various tough plan materials and easily scales for labs looking for high-throughput solutions. In our study, the Omni Bead Ruptor bead mill homogenizer was used to homogenize and extract DNA from frozen spinach leaves and okra pods. Both sample types – soft and fibrous plant tissue – were sufficiently homogenized in a one minute cycle at 4 m/s to take into DNA extraction, resulting in quantities of gDNA cleanly visible by 1D gel electrophoresis, and indicative of high DNA integrity suitable for downstream genomic applications. For research use only. Not for use in diagnostic procedures.

Genomics and Epigenomics research sample prep automation workflow flyer

Corn is an important stable crop, not only as a food source for humans and livestock, but also biofuel as well other non-food products. This versability makes corn an importance resource and topic of research globally. Within agricultural science and sustainability, labs are hard at work better understanding the genetic mechanisms behind healthy growth and environmental adaptability, whether in response to factors such as climate change, availability of arable land, susceptibility to disease. With the adoption of high-throughput sequencing assessing plant transcriptomics, labs require sample prep methods that reliably allows the extraction of RNA with high integrity for downstream assays, which can be challenging due to thick polysaccharide cell walls and the layers inherent to certain sample types. Semi-automated tabletop bead mills allow for repeatability only afforded by automation-enabled techniques that feed into automated nucleic acid extractions that scales as your throughput increases. For research use only. Not for use in diagnostic procedures.

Bats are essential to the biodiversity critical to sustaining ecosystems, and at the same time, they are also widely studied because they are capable of transmitting viruses to the general human population. Both ecological and animal scientists and human disease researchers depend on reliable sample processing solutions such as bead mills to process tough samples in a safe manner. We evaluate protocols utilizing bead milling in sealed tubes, which helps minimize both risk of dispersion of potentially hazardous materials and risk of cross-contamination without diminishing efficiency, via the Omni Bead Ruptor Elite bead mill homogenizer to process bat fur and skin via both dry-grinding and wet-grinding methods.

Halal and Kosher dietary laws prohibit the use of products containing porcine origin. Pork-based additives, such as collagens used in gelatin or as gelling agents in various processes, are surprisingly common in a variety of food and pharmaceuticals. Bead milling, such as with the Omni Bead Ruptor Elite, offers a high-throughput solution for homogenizing samples for DNA or protein extraction. Methods such as PCR or LC-MS/MS are conductive and crucial for detecting porcine-derived collagen. Bead milling using single-use, sealed tubes are particularly effective for challenging confectionery matrices, and requires no hazardous cleaning solutions; it also offers an alternative that significantly reduces processing time compared to single-sample, manual methods. Herein, we provide recommended parameters for bead mill homogenization of candy.

Metagenomics and microbial studies sample prep workflow flyer

Cannabinoid quantification is a common analytical method used by many cannabis producers and testing facilities to define the quantity of cannabinoids in their plants and plant-derived products. The potency of cannabis is typically expressed as the sum of acidic and neutral forms of cannabinoids, with the plant primarily synthesizing the carboxylic acid forms. These forms, however, convert to their neutral counterparts when exposed to substantial heat, such as in the process of smoking or general cannabinoid-containing food production. As such, it is important to maintain this ratio of neutral/acid cannabinoid forms during the sample prep process through analysis. Common methods include manual hand-grinding, rotor-stator homogenization, as well as bead- or cryo-milling. In this study, the effect of bead milling on sample temperature and acidic cannabinoid decarboxylation was evaluated. Cannabinoid standards for THC, THCA, CBD, and CBDA were prepared and serially diluted into a series ranging from 250 ppm (stock) to 7.81 ppm. Samples were analyzed using reverse phase HPLC, where peak areas were integrated for each standard and compared against known concentrations. The degree of decarboxylation was assessed using relative analyte concentrations, and relative amount expressed as a percentage. Results from the parameters evaluated indicate that the Omni Bead Ruptor Elite homogenizer processes cannabinoids without causing decarboxylation, maintaining the ratio of neutral/acid cannabinoid forms for proper quantification

In biomedical research, the ability to reliably extract DNA and RNA from fresh tissue samples is important for molecular applications. It provides an efficient alternative to traditional and manual methods, reducing total processing time and helping with sample-to-sample consistency. We demonstrate the use of the Omni Bead Ruptor Elite™ bead mill homogenizer with stainless-steel lysing/grinding cylinders to yield robust concentrations of DNA and RNA, suitable for downstream molecular applications. The stainless-steel lysing/grinding cylinders are durable and can also facilitate cryogenic sample preparation.

In this study, the Omni Bead Ruptor Elite homogenizer was integrated into a semi-automated workflow for high-throughput nucleic acid extraction from various mouse tissues, including liver, kidney, heart, skin, skeletal muscle, and lungs. An efficient protocol using off-the-shelf chemistry was developed and our results demonstrate its robust scalability, where a processing range from 10 – 100 mg showed proportional increases in nucleic acid yield for most tissue types with this bead mill and KingFisher™ Flex system combination. While liver and kidney samples showed some saturation effects at higher masses, the homogenization parameters and the extraction protocol employed, generally produced nucleic acids that could be reliably feed into common downstream applications such as PCR and sequencing. The workflow, therefore, offers a reliable solution and path to automation for researchers working with multiple types of complex tissue and looking to improve laboratory efficiency. For research use only. Not for use in diagnostic procedures.

Omni BR Cryo Cooling Unit product marketing sheet

The QuEChERS method, a solvent extraction technique introduced in 2003, is widely used for the extraction of multiple pesticides from samples; It has since been optimized to support a broad range of analytes. It starts with the comminution of samples, which can include the convenient and reliable use of bead-beating in solvent. This study describes the application of bead mill homogenization to simultaneously homogenize several samples for QuEChERS extraction for pesticide detection in common fruits and vegetables, with full homogenization achieved in 30 seconds for all assayed produce types in 50 mL tubes. Pesticides were then extracted and analyzed by LC-MS/MS.

Disease modelling and the evaluation of toxicity and safety of potential therapies involve the use of common preclinical animals, including mice. As the field continues to progress in fine-tuning the responsible use of animal models together with adoption of technologies such as OOCs or 3D models, a standardized sample prep process is valuable in multiomic profiling of animal tissues for pre-clinical stages of research, drug discovery, and drug development to help the universal goal of lowering the attrition rate of drug candidates. We evaluated the Omni Bead Ruptor Elite bead mill homogenizer for mechanical disruption as a unifying platform for upstream sample homogenization from various relevant tissues for further downstream processes, including protein analysis via immunoassays and genomic applications such as qPCR. Studies in review within this poster include the following: Study 1: High-throughput RNA extraction from mouse kidney, liver, heart, and lung using bead milling vs manual mortar & pestle Study 3: Homogenization and protein extraction to assess kidney toxicity in mice treated with antimetabolic agents

Induced pluripotent stem cell (iPSC) research has allowed countless insights into biological processes in homeostasis and disease progression. To obtain a comprehensive view of relevant genes and their expression profiles, robust upstream tissue homogenization is an integral part of the workflow. We evaluated bead mill homogenization via the Omni Bead Ruptor Elite bead mill homogenizer to assess efficacy in preparing iPSC-derived neural progenitor (NP) cells for RNA isolation and subsequent COX-1 and COX-2 detection.

Cytokine Release Syndrome (CRS) can lead to significant tissue damage, and it is an active field of immunology research. We outline a workflow for tissue lysate preparation and quantification of 13 key biomarkers relevant to CRS using the LEGENDplex™ panels and the Omni Bead Ruptor Elite homogenizer. The results show that bead mill homogenization is effective in disrupting tissues for cytokine quantification to support research on inflammatory processes.

Application note quantifying immune mediators from serum and skin tissue using LPS-induced murine model of inflammation via multi-sample processing.

Reliable extraction of viral RNA from tissue samples is an important step in viral detection for research studies. The Omni Bead Ruptor Elite bead mill homogenizer helps reduce the time needed for initial homogenization steps upstream RNA kit extractions, allowing simultaneous processing of up to 24 samples in 30 seconds in this proof-of-concept study using viral cultures. The protocol using bead mill homogenization upstream of sample vortexing demonstrated increased efficiency over traditional vortexing methods alone. The Omni Bead Ruptor Elite bead mill homogenizer is recommended for its increased throughput, robust RNA yields, and decreased time required for extractions of viral RNA from multiple samples. For research use only. Not for use in diagnostic procedures.