Newest Assay Kits

Alpha technolgy enables the rapid and straightforward mesaure of virtually any target. This includes enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules. Explore the vast selection of homogenous Alpha assays and reagents, including AlphaLISA ™ , AlphaLISA ™ SureFire ® Ultra ™ , AlphaScreen ™ , and Alpha Toolbox. For research use only. Not for use in diagnostic procedures.

Discover Alpha SureFire ®   Ultra ™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology and sandwich immunoassays for detecting phosphorylated proteins in cells. Offering a quantitative alternative to Western Blotting, Alpha SureFire assays are automation-friendly, easily miniaturized, and proficient in detecting both endogenous and recombinant proteins. Explore our comprehensive portfolio of SureFire Assays, designed to help you elevate and expedite your drug discovery journey.

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay Several biological processes are regulated by protein phosphorylation. It is, therefore, no surprise that the dysregulation of protein phosphorylation is implicated in a relatively large number of diseases. AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments. This guide contains tools and data helpful for you to perform your assays using AlphaLISA SureFire Ultra: A detailed description of the assay and its options A thorough investigation of assay conditions to obtain the optimal response from the chosen modulator and cell line A list of optimization steps to provide a sufficient assay window and produce the strongest results possible

pHSense™ probes are plate reader-compatible reagents with timeresolved fluorescence (TRF) readout specifically developed for internalization studies. Fully compatible with our plate reader platforms, this technology reinforces Revvity’s commitment to advancing drug discovery workflows. pHSense offers: Higher throughput plate-based pH sensitive internalization reagents Superior signal-to-background for more reliable pharmacological data Versatility across suspension & adherent cells For research use only. Not for use in diagnostic procedures.

pHSense™ probes are plate reader-compatible reagents with time-resolved fluorescence (TRF) readout specifically developed for internalization studies. Fully compatible with our plate reader platforms, this technology reinforces Revvity’s commitment to advancing drug discovery workflows. pHSense offers: Higher throughput plate-based pH sensitive internalization reagents Compatibility with standard plate readers Superior sensitivity at low endogenous expression levels For research use only. Not for use in diagnostic procedures.

Antibody internalization is a critical step for evaluating the efficacy of therapeutic antibodies and antibody-drug conjugates (ADCs). Accurate detection of this process is essential for understanding mechanism of action, optimizing design, and accelerating drug development. This technical note introduces pHSense™ Eu Fab reagents, Europium-labeled, pH-sensitive probes designed for no-wash, live-cell assays using time-resolved fluorescence (TRF). These reagents enable sensitive detection of antibody and ADC internalization while minimizing background signal. Learn how to: Monitor internalization of antibodies and ADCs in HER2 and EGFR-expressing cell models Achieve reliable EC₅₀ values using live-cell, no-wash assays Validate assay performance across pH ranges and IgG isotypes Explore how pHSense probes support high-throughput internalization studies and empower antibody-based therapeutic development. For research use only. Not for use in diagnostic procedures.

This techincal note explores how pHSense™ Eu TRF probes enable no-wash, live-cell detection of GPCR internalization across overexpressed and endogenous models, with high sensitivity and low background.

Discover pHSense™ probes – plate reader-compatible reagents with time-resolved fluorescence (TRF) readout, specifically developed to advance cellular internalization studies. This catalog includes: Introduction to pHSense probes Applications in ADC and GPCR research Comprehensive list of available products For research use only. Not for use in diagnostic procedures.

This publication review highlights the work of Trist et al. about the characterization of Alpha-Synuclein pathology in preclinical models using AlphaLISA SureFire® Ultra assays. This publication review details: Ultra-sensitive detection of α-synuclein and pS129 in mouse and human samples Validated performance across tissues and cell models with minimal background Clear link between phosphorylation and aggregation in diseased brain regions Scalable platform for biomarker discovery and therapeutic research in synucleinopathies For research use only. Not for use in diagnostic procedures.

Discover how Revvity's integrated immunoassay ecosystem enhances research consistency, efficiency, and data quality.

This document includes detailed tables listing HTRF™, AlphaLISA™ SureFire® Ultra™, and Alpha SureFire® Ultra™ Multiplex assays. Each assay is evaluated for its compatibility with species including Human, Mouse, Rat, Monkey, Hamster, Drosophila, Pig, and Dog. For research use only. Not for use in diagnostic procedures.