Microplates for Storage

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody. Alpha technology offers a simple, straight forward workflow. No wash needed! Add sample Add Acceptor bead mix; incubate 1 hr Add Donor beads; incubate 30 mins Read on Alpha-enable microplate reader Download this application note to see how no-wash AlphaLISA ™ technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

Your specific applications deserve the best microplates! For a researcher, the need to select the most suitable application-specific microplates is critical. Format, color, and surface properties are among the different features that can impact your assay performances and the quality of your results. Covering a vast array of application areas and over many years, Revvity has been committed to offering you high quality plates for the highest quality results. Download this guide and let us walk you through the process of choosing the correct microplate for your specific application.

Here we demonstrate the ease and versatility of using LANCE Ultra TR-FRET assays to quantitate low levels of the pro-inflammatory cytokines TNFα, IL-6, and IL-1β from PMA differentiated U937 cell supernatants and to detect measurable inhibition of cytokine secretion using the small molecule dexamethasone.

Antibody-dependent cellular cytotoxicity (ADCC) is a cell-mediated immune response where an effector cell destroys an antibody-bound target cell. This process involves cells that express tumor or pathogen-derived antigens on their surface, which are targeted by therapeutic antibodies. The binding of the antibody and the effector cell triggers a series of signaling events, leading to the activation of the effector cell and the release of chemokines and cytokines into the tumor microenvironment. Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies. Read this application note to access: ADCC assay protocol - ADCC experiment with rituximab Data collection and analysis Formula Calculation and detailed results