Microplates for Genomic Analysis

Leptospirosis is an infectious disease caused by Leptospira bacteria, which can spread across different species of mammals. Transmission occurs through contact or consumption of food from infected domestic animals. In cattle, Leptospira infection leads to reproductive failure, affecting productivity and profitability in the dairy and beef industries. Vaccinations are widely used to prevent infection in animals and reduce transmission to humans. This application note: demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigen stimulated PBMCs isolated from cow blood shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody. Alpha technology offers a simple, straight forward workflow. No wash needed! Add sample Add Acceptor bead mix; incubate 1 hr Add Donor beads; incubate 30 mins Read on Alpha-enable microplate reader Download this application note to see how no-wash AlphaLISA ™ technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

G protein-coupled receptors (GPCRs) are a large family of transmembrane receptors that are significant drug targets. They function as enzymes that activate G-proteins. GPCRs couple to three main families of Gα subunits: Gαi/o, Gαs, and Gαq. The coupling specificity depends on the GPCR and its cellular environment. Gαs and Gαi subunits operate through the cAMP pathway, either activating or inhibiting adenylate cyclase, which converts ATP to cAMP. In this application note, the AlphaScreen™-based cAMP detection technology has been used to study Gαi-coupled receptors, specifically the 5-hydroxytryptamine-1A (5-HT1A) serotonergic receptor. The note demonstrates the utility of the AlphaScreen-based cAMP Assay kit for the pharmacological characterization and potential high-throughput screening of Gαi-coupled receptors.

G protein-coupled receptors (GPCRs) play a crucial role in various cellular and physiological processes, including cell proliferation, differentiation, neurotransmission, development, and apoptosis. GPCR activity is commonly assessed by measuring levels of intracellular cAMP upon stimulation by agonists. Abnormal GPCR activity is associated with many diseases, including cancer, making GPCRs an important class of pharmaceutical drug targets. Multiple assay formats have been developed to measure the level of cAMP generated, which can determine the pharmacological potency of different agonists and antagonists. The ideal assay is homogeneous, non-radioactive, and allows for sensitive and reproducible detection. AlphaScreen ™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research. Download this application note to learn more on the cAMP AlphaScreen assay: Detailed protocol, Data illustrated by graph and tables, Dynamic range description and validation.

Mouse pharmacological models continue to play a large role in the study of human disease, and mouse tool reagents have shown high utility in immunology and cancer research for decades. It can often be quicker to learn about immunology and the regulation of immune responses using a syngeneic mouse model. However, working in mouse systems can often require the development of separate mouse reagents, if the therapeutic agent of interest does not cross-react with mouse. Find out how the AlphaLISA™ human PD-1/PD-L1 and AlphaLISA mouse PD-1/PD-L1 binding assays provide a fast, powerful, homogeneous platform for obtain binding potencies from potential novel drug candidates.

Your specific applications deserve the best microplates! For a researcher, the need to select the most suitable application-specific microplates is critical. Format, color, and surface properties are among the different features that can impact your assay performances and the quality of your results. Covering a vast array of application areas and over many years, Revvity has been committed to offering you high quality plates for the highest quality results. Download this guide and let us walk you through the process of choosing the correct microplate for your specific application.

Over the last decade, therapeutic antibodies have emerged as the predominant class of new drugs. Assays to functionally characterize and evaluate antibodies can be used in the discovery process as well as during the development stages of antibody therapeutics. In this detailed case study, we show a suite of assays that can be used to compare an engineered antibody (adalimumab Fc Silent ™ ) to the original therapeutic antibody (Adalimumab). In this application note, discover how Alpha, HTRF and LabChip ® Protein Express assays can help when you are: Comparing the binding affinities of engineered antibody or original therapeutic antibody to the target or Fc receptors, or for readouts in functional cell-based assays Assessing binding capabilities after thermal stress, to check antibody stability Looking for critical data to build confidence in a biosimilar or an engineered therapeutic antibody

Here we demonstrate the ease and versatility of using LANCE Ultra TR-FRET assays to quantitate low levels of the pro-inflammatory cytokines TNFα, IL-6, and IL-1β from PMA differentiated U937 cell supernatants and to detect measurable inhibition of cytokine secretion using the small molecule dexamethasone.

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with several distinct advantages over ELISA technology.

Measuring low levels of biomarkers in complex biological samples such as serum and plasma is always a challenge. This is further complicated by the low sample volumes collected from mice, which are widely used in the early stages of drug discovery and development. This application note demonstrates the quantification of low levels of mIL-17A homodimer in mouse serum and plasma samples using the AlphaLISA ® mIL-17A homodimer detection kit. Key features: Detailed Material and Methods Multiple matrices and assay formats assessed Exhaustive results

Antibody-dependent cellular cytotoxicity (ADCC) is a cell-mediated immune response where an effector cell destroys an antibody-bound target cell. This process involves cells that express tumor or pathogen-derived antigens on their surface, which are targeted by therapeutic antibodies. The binding of the antibody and the effector cell triggers a series of signaling events, leading to the activation of the effector cell and the release of chemokines and cytokines into the tumor microenvironment. Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies. Read this application note to access: ADCC assay protocol - ADCC experiment with rituximab Data collection and analysis Formula Calculation and detailed results

Various cytokines are secreted during an active immune response that can have modulatory effects on target cell populations, including interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNFa) and several interleukins. In this application note, you will learn how we investigated: The effects of secreted factors on a triple-negative breast cancer cell line by treatment with conditioned media from activated PBMCs Whether the modulatory effects of cytokines secreted by infiltrating immune is different when studied in 3D cell cultures How to detect and quantify multiple immune-modulated proteins from the same wells in complex cell models grown in 3D spheroid microplates and traditional 2D cell culture using AlphaLISA ™ technology and EnVision ® multimode plate reader in a workflow with ATPlite® 1step and Opera ® Phenix ® high-content screening system

Asthma is a chronic inflammatory disease that affects millions of people around the world. The detection of specific asthma biomarkers in serum can help diagnose and monitor the disease. AlphaLISA is a bead-based assay technology for the detection of these molecules. This technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα. Read this application note to: .discover in detail how AlphaLISA technology can be used to quantify biomarker level in serum samples, ensure proper and accurate detection of biomarkers in serum, dilution linearity and spike-and-recovery experiments to determine the proper diluents and dilution factors required for accurate recovery.