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    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
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        • qPCR
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        • Viral Vector Products
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      • Protein Analysis
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        • Recombinant Proteins
        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
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      • Cell Analysis
        • Cell Isolation
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        • Microplates
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      • Research Solutions
        • Immunoassays Solutions
        • pHSense Reagents
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        • GMP Workflows
        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Physiological Model Solutions
        • Biobanking Workflows
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    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
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      • Allergy
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      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

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      • Rapid Patient Testing
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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
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      • Cellular Imaging Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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      • Immunoassays
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      • In Vivo Imaging Reagents
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      • Liquid Scintillation Cocktails
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      • pHSense Reagents
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      • qPCR
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    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Automated Liquid Handling
        • Flow Cytometry Cocktail Prep Automation
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Sample Homogenization
        • Homogenizers
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        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
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      • Consumables & Accessories
        • Cassettes
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        • High-Content Imaging Accessories
        • Microplates
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        • Scintillation Cocktails
        • Transfer Membranes
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        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

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      • Instrument Service & Maintenance
        • On Demand Equipment Service
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        New! Cellometer™ Ascend™

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      New! Cellometer™ Ascend™

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    • Consumables & Accessories
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    • Signals Software
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      • All Solutions
        • Biology
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Panel Screening
        Preclinical services

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      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
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        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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  • Company
    • Purpose
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    • Investor Relations
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        Investor Day 2024

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      Investor Day 2024

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  • Brands
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    • BioLegend

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    • Revvity Omics

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    • Tulip Diagnostics

    • ViaCord

    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

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  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

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    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

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GPCR Cell Lines

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From GPCR cell lines to luciferase-labeled tumor cells for in vivo imaging studies, to engineered cells we offer a wide range of cell lines designed to fit your research and development needs.

GPCR cell lines: Over 300 stable GPCR cells lines for binding and functional testing and over 130 cells lines for calcium testing

  • AequoScreen® cell lines are doubly transfected to co-express both Aequorin and a variety of GPCRs for calcium flux assays
  • ValiScreen® cell lines express a variety of different GPCRs
  • PhotoScreen® cell lines include photoprotein kinetic luminescence technology for measuring calcium flux in cells

Luciferase-labeled tumor cell lines: IVISbrite™ bioluminescent tumor cells, for in vivo imaging, are labeled with red-shifted luciferase that offer a brighter signal

Engineered cell lines: With over 7,500 ready-to-go' cell lines to custom services, Horizon Discovery has the expertise to provide unrivaled cell line engineering solutions

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Tag-lite® binding assays

Revvity's Tag-lite binding assays offer straightforward add-and-read protocols to help you characterize the binding properties of compounds, regardless of their chemical structures (peptides, small or complex molecules), or pharmacological properties (agonist, antagonist, or inverse agonist).

Revvity's Tag-lite binding assays offer straightforward add-and-read protocols to help you characterize the binding properties of compounds, regardless of their chemical structures (peptides, small or complex molecules), or pharmacological properties (agonist, antagonist, or inverse agonist).

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Radioligand selection guide

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1 - 25 of 265 Products and services
GPCR Receptor Cells and Membranes
ValiScreen Human Adrenergic α2B Cell Line, CHO-K1 Cells

Stable, recombinant ValiScreen® cell line expressing α2B Adrenergic receptor, human recombinant in CHO-K1 host cells.

Part number: ES-031-C
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GPCR Receptor Cells and Membranes
ValiScreen Human Chemokine CCR1 Cell Line, CHO-K1 Cells

Stable, recombinant ValiScreen® cell line expressing the Chemokine receptor CCR1, human recombinant in CHO-K1 host cells.

Part number: ES-132-C
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GPCR Receptor Cells and Membranes
AequoScreen Human Histamine H2 Cell Line, CHO-K1 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and the Histamine H2 receptor, human recombinant in CHO-K1 host cells.

Part number: ES-391-A
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GPCR Receptor Cells and Membranes
AequoScreen Parental Cell Line, Gα16, CHO-K1 Cells

Stable, recombinant AequoScreen® parental cell line expressing aequorin and Gα16 in CHO-K1 host cells.

Part number: ES-000-A24
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GPCR Receptor Cells and Membranes
AequoScreen Human Free Fatty Acid FFA (GPR120) Cell Line, CHO-K1 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and the Free fatty acid receptor FFA (GPR120), human recombinant in CHO-K1 host cells.

Part number: ES-800-A
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GPCR Receptor Cells and Membranes
PhotoScreen Human Recombinant Purinergic P2RX4, HEK293 Cell Line

Stable, recombinant PhotoScreen® cell line expressing a photoprotein and the P2X purinoceptor 4 (P2RX4), human recombinant in HEK293 host cells.

Part number: AX-015-PCF
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GPCR Receptor Cells and Membranes
AequoScreen Parental Cell Line, HEK293 Cells

Stable, recombinant AequoScreen® parental cell line expressing aequorin in HEK293 host cells.

Part number: ES-000-A30
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GPCR Receptor Cells and Membranes
AequoScreen Human Serotonin 5-HT2C (edited) Cell Line, CHO-K1 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and the Serotonin receptor 5-HT2C, human recombinant in CHO-K1 host cells.

Part number: ES-315-A
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GPCR Receptor Cells and Membranes
AequoScreen Human Glutamate (mGlu5a) Cell Line, CHO-K1 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and theGlutamate receptor mGlu5A, human recombinant in CHO-K1 host cells.

Part number: ES-555-A
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GPCR Receptor Cells and Membranes
AequoScreen Human Serotonin 5-HT3A Cell Line, HEK293 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and the Serotonin receptor 5-HT3A, human recombinant in HEK293 host cells.

Part number: ES-402-A
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GPCR Receptor Cells and Membranes
Norepinephrine Transporter (human) Cell Line

Stable, recombinant ValiScreen® cell line expressing the Norepinephrine transporter receptor, human recombinant in MDCK host cells.

Part number: RBHNETM-K
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GPCR Receptor Cells and Membranes
ValiScreen Human Recombinant Transient Receptor Protein 3 (TRP-3), HEK293 Cell Line

Stable, recombinant ValiScreen® cell line expressing Short transient receptor potential channel 3 (Trp3), human recombinant in HEK293 host cells.

Part number: AX-011-C
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GPCR Receptor Cells and Membranes
Dopamine Transporter (human) Cell Line

Stable, recombinant ValiScreen® cell line expressing the Dopamine transporter receptor, human recombinant in CHO-K1 host cells.

Part number: RBHDATM-K
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GPCR Receptor Cells and Membranes
AequoScreen Parental Cell Line, Gα16, medium level, CHO-K1 Cells

Stable, recombinant AequoScreen® parental cell line expressing aequorin and Gα16 (medium level) in CHO-K1 host cells.

Part number: ES-000-A2
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GPCR Receptor Cells and Membranes
AequoScreen Cells, opioid kappa receptor, human recombinant, HEK293

Stable, recombinant AequoScreen® cell line expressing aequorin and the Opioid kappa receptor, human recombinant in HEK293 host cells.

Part number: 6110558-K
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GPCR Receptor Cells and Membranes
ValiScreen Cells, opioid delta receptor, human recombinant, CHO-K1

Stable, recombinant ValiScreen® cell line expressing the Opioid delta receptor, human recombinant in CHO-K1 host cells.

Part number: RBHODM-K
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GPCR Receptor Cells and Membranes
AequoScreen Parental Cell Line, Gα16, HEK293 Cells

Stable, recombinant AequoScreen® parental cell line expressing aequorin and Gα16 in HEK293 host cells.

Part number: ES-000-A26
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GPCR Receptor Cells and Membranes
Valiscreen Cells, opioid delta receptor, human recombinant, HEK293

Stable, recombinant Valiscreen cell line expressing aequorin and the Opioid delta receptor, human recombinant in HEK293 host cells.

Part number: 6110549-K
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GPCR Receptor Cells and Membranes
ValiScreen Human Recombinant Transient Receptor Protein 6 (TRP-6), HEK293 Cell Line

Stable, recombinant ValiScreen® cell line expressing Short transient receptor potential channel 6 (Trp6), human recombinant in HEK293 host cells.

Part number: AX-012-C
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GPCR Receptor Cells and Membranes
ValiScreen Human Muscarinic M2 Cell Line, CHO-K1 Cells

Stable, recombinant ValiScreen® cell line expressing the Muscarinic acetylcholine M2 receptor, human recombinant in CHO-K1 host cells.

Part number: ES-211-C
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GPCR Receptor Cells and Membranes
ValiScreen Human Cholecystokinin CCKCCK2 Cell Line, 1321N1 Cells

Stable, recombinant ValiScreen® cell line expressing the Cholecystokinin receptor CCK2, human recombinant in 1321N1 host cells.

Part number: ES-531-C
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GPCR Receptor Cells and Membranes
PhotoScreen Human Recombinant Urotensine UT (Uro II, GPR14), CHO-K1 Cell Line

Stable, recombinant PhotoScreen® cell line expressing a photoprotein and the rotensin receptor UT (Uro II, GPR14), human recombinant in CHO-K1 host cells.

Part number: AX-007-PCF
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GPCR Receptor Cells and Membranes
ValiScreen Human Chemokine CXCR3 Cell Line, CHO-K1 Cells

Stable, recombinant ValiScreen® cell line expressing the Chemokine receptor CXCR3, human recombinant in CHO-K1 host cells.

Part number: ES-142-C
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GPCR Receptor Cells and Membranes
ValiScreen Human Neuropeptide FF Receptor 1 (NPFF1) Cell Line, CHO-K1 Cells

Stable, recombinant ValiScreen® cell line expressing the Neuropeptide FF receptor 1 (NPFF1) receptor, human recombinant in CHO-K1 host cells.

Part number: ES-491-C
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GPCR Receptor Cells and Membranes
AequoScreen Human Chemokine CCR3 Cell Line, CHO-K1 Cells

Stable, recombinant AequoScreen® cell line expressing aequorin and the Chemokine receptor CCR3, human recombinant in CHO-K1 host cells.

Part number: ES-138-A
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Application Note
Application Note
cAMP AlphaScreen assay: a method for the pharmacological characterization and screening of Gαi coupled receptors in whole cells

G protein-coupled receptors (GPCRs) are a large family of transmembrane receptors that are significant drug targets. They function as enzymes that activate G-proteins. GPCRs couple to three main families of Gα subunits: Gαi/o, Gαs, and Gαq. The coupling specificity depends on the GPCR and its cellular environment. Gαs and Gαi subunits operate through the cAMP pathway, either activating or inhibiting adenylate cyclase, which converts ATP to cAMP. In this application note, the AlphaScreen™-based cAMP detection technology has been used to study Gαi-coupled receptors, specifically the 5-hydroxytryptamine-1A (5-HT1A) serotonergic receptor. The note demonstrates the utility of the AlphaScreen-based cAMP Assay kit for the pharmacological characterization and potential high-throughput screening of Gαi-coupled receptors.

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Application Note
Application Note
cAMP assay provides flexibility and stable pharmacology

G protein-coupled receptors (GPCRs) play a crucial role in various cellular and physiological processes, including cell proliferation, differentiation, neurotransmission, development, and apoptosis. GPCR activity is commonly assessed by measuring levels of intracellular cAMP upon stimulation by agonists. Abnormal GPCR activity is associated with many diseases, including cancer, making GPCRs an important class of pharmaceutical drug targets. Multiple assay formats have been developed to measure the level of cAMP generated, which can determine the pharmacological potency of different agonists and antagonists. The ideal assay is homogeneous, non-radioactive, and allows for sensitive and reproducible detection. AlphaScreen ™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research. Download this application note to learn more on the cAMP AlphaScreen assay: Detailed protocol, Data illustrated by graph and tables, Dynamic range description and validation.

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Application Note
Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies and small molecules. CCR7 and CXCR2 receptors, which are expressed in immune cells and are therapeutic targets for disorders like lupus erythematosus, adult leukemia, lymphomas, chronic obstructive pulmonary disease (COPD), and sepsis. AlphaLISA ™ SureFire ® Ultra ™ and Alpha SureFire ® Ultra ™ Multiplex assays are automation-friendly, applicable to both small and large-scale screens, and can assess phosphorylation status in complex matrices. The LANCE Ultra cAMP assay is measures cyclic AMP (cAMP) produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs). This application note demonstrates how the SureFire Ultra and LANCE Ultra cAMP assays can be used for measuring inhibitors to CCR7 and CXCR2 cell surface receptors using a cellular model system where these receptors are overexpressed in CHO cells. The assays were optimized to measure receptor blockage and assayed receptor activity modulation by detecting ERK and AKT phosphorylation status and cAMP modulation. For more details, download the application note!

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Application Note
Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit

G protein-coupled receptors (GPCRs) are cell surface transmembrane receptors that activate G-proteins. GPCR activity is often assessed by measuring intracellular cAMP levels upon stimulation by agonists. Since GPCRs are important pharmaceutical drug targets, there’s a high demand for methods to detect and quantify cAMP. The ideal assay is homogenous, non-radioactive, and allows for sensitive and reproducible detection of cAMP. The AlphaScreen™ cAMP assay is one such method. It was evaluated for its ability to detect agonist or antagonist-induced cAMP responses in cells expressing either endogenous or recombinant receptors. Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit.

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Brochure
Brochure
GPCRs: the pathway to discovery

G protein coupled-receptors (GPCRs) are one of the most intensively studied drug targets, with up to one-third of all marketed therapeutics acting by binding to GPCRs. Therapies that target GPCRs include both agonist and antagonists that are used in the treatment of disease in nearly every major organ system and hold significance in several therapeutic areas, such as cardiovascular diseases, neurosciences, respiratory diseases, metabolic disorders, oncology, and rare diseases. We know the importance of furthering GPCR research - and we answer the call with application notes, white papers, and end-to-end solutions including reagents, instruments, and tools for all aspects of research into today’s most promising drug target. For research use only. Not for use in diagnostic procedures.

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Literature - Publication Review
Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments

Liquid tumors, which occur in the blood, bone marrow, or lymph nodes, are categorized into three main types of cancer: leukemia, lymphoma, and myeloma. Unlike solid tumors such as carcinomas and sarcomas, these cancers do not form solid masses, leading to different diagnosis and treatment approaches. Leukemia and lymphoma, both forms of blood cancer, affect the body differently. Leukemia primarily impacts the blood and bone marrow, while lymphomas mainly affect the lymph nodes. Significant progress has been made in developing new treatments for both cancers, often targeting the signaling pathways that are constantly activated in specific cancer types. This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.

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Application Note
Application Note
LANCE Ultra cAMP: a new TR-FRET cAMP assay for Gs - and Gi-coupled receptors.

We have developed a second-generation LANCE® time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated GPCRs

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Application Note
Application Note
Measuring Performance of an Automated and Miniaturized LANCE Ultra cAMP Assay for the Gi-coupled 5-HT1A Receptor- a Comparative Study

In this note we describe the miniaturization of a LANCE Ultra cAMP assay from 384-to 1,536-well plate format for the identification and characterization of agonists and antagonists of a Gi-coupled GPCR.

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Technical Note
Technical Note
phsense eu probes time-resolved fluorescence plate based applications for monitoring internalization of overexpressed and endogenous GPCRs

This techincal note explores how pHSense™ Eu TRF probes enable no-wash, live-cell detection of GPCR internalization across overexpressed and endogenous models, with high sensitivity and low background.

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Brochure
Brochure
Radioligand selection guide

Discover a comprehensive collection of GPCR cell lines, membrane preparations, and complementary radioligands designed to drive your GPCR research. Utilize this selection guide to easily identify: Cell lines available for specific receptors Assays that cell lines are validated for Corresponding complementary radioligands and their pharmacological action Learn more about the tools to support your research in this guide!

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