ctDNA Workflows

We employ cutting-edge technologies to allow for comprehensive genetic profiling with an accelerated workflow and minimal hands-on time. Use as little as 10 ng of input cfDNA for your library preparation using the NEXTFLEX™ Cell Free DNA-Seq Kit 2.0.

Suggested products:

• NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0

Verify your oncology workflows with our cell line-derived Mimix™ Cell-Free DNA reference standard for patient-like performance. Containing fragmented DNA to mimic ctDNA with genetically defined common cancer mutations providing reliable controls for liquid biopsy assays.

• Mimix Cell-free DNA Reference Standard

By incorporating unique dual indexes (UDI) into our adapters, Revvity ensures that each sample is uniquely identified during sequencing, which helps prevent index hopping and cross-contamination. We also offer adapters with both unique molecular identifiers (UMI). UMIs are used to track individual DNA or RNA molecules throughout the sequencing process. This is especially useful for improving data accuracy. Both NEXTFLEX UDI-UMI and UDI barcodes facilitate high-throughput multiplexing, reduction of false positive calls and rare variant detection.

• NEXTFLEX Adapters

Globin transcripts constitute a significant portion of the total mRNA in whole blood or blood-derived samples such as plasma. This can compromise the detection of low-abundance transcripts. The DepleteX™ Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.

• DepleteX Globin Depletion Kit