Skip to main content
Menu
Revvity logo
Contact us
  • Request a quote
  • Contact sales
  • Contact customer care
  • Application support
  • Instrument service request
  • Training request
US
Search all
  • Who we serve
    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

      Visit BioLegend.com

    • Pharma / Biotech
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Clinical Laboratories
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Healthcare Professionals
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Contract Research Organizations
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
        • cDNAs and ORF Clones
        • Single-Cell Sequencing
        • Labeled Nucleotides
        • qPCR
        • Library Prep Kits
        • Magnetic Beads
        • Viral Vector Products
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Protein Analysis
        • Binding Assays
        • Immunoassays
        • Microfluidic Protein Characterization
        • Microplate Readers
        • Microplates
        • Recombinant Proteins
        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Cell Analysis
        • Cell Isolation
        • Cell Lines & Stem Cells
        • Cell Counting and Image Cytometry
        • Cell Health & Viability
        • Cellular Imaging & Analysis
        • Immunoassays
        • In Vivo Imaging
        • Microplates
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Research Solutions
        • Immunoassays Solutions
        • pHSense Reagents
        • Biomarker Discovery
        • Cell and Gene Therapy
        • GMP Workflows
        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Physiological Model Solutions
        • Biobanking Workflows
        • Agrigenomics Workflows
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

        Learn more

      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Endocrinology
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Allergy
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      • Rapid Patient Testing
        New IVD Mimix™ reference standards for oncology workflows.

        Learn more

      New IVD Mimix™ reference standards for oncology workflows.

      Learn more

    • Reagents
      • BioLegend
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Cellular Imaging Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • chemagic Nucleic Acid Purification Kits
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • CRISPR Technologies
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • GPCR Research Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Immunoassays
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • In Vivo Imaging Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Liquid Scintillation Cocktails
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • NGS Library Prep Kits
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • pHSense Reagents
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • qPCR
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Radiochemicals
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • RNA Interference
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Primary and Secondary Antibodies
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Automated Liquid Handling
        • Flow Cytometry Cocktail Prep Automation
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Sample Homogenization
        • Homogenizers
        • Homogenizer Accessories
        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Consumables & Accessories
        • Cassettes
        • Cell Harvesters
        • In Vivo Imaging Accessories
        • High-Content Imaging Accessories
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
        • Transfer Membranes
        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      • Instrument Service & Maintenance
        • On Demand Equipment Service
        • Equipment Service Plans
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

        Learn more

      New! Cellometer™ Ascend™

      Explore our cell counting and image cytometry solutions.

      Learn more

    • Consumables & Accessories
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Signals Software
      • All Products
        • Signals One
        • ChemDraw
        • Spotfire®
        • Signals Clinical
        • Signals Notebook
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • All Solutions
        • Biology
        • Discovery Chemistry
        • Clinical & Translational
        • Specialty Chemicals
        • Food/Flavor/Fragrance
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Revvity Omics Services
      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Cell Panel Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

        Learn more

      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

      Learn more

    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Gene Expression Systems
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

        Learn more

      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

      Learn more

    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

        Learn more

      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

      Learn more

    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

    Learn more

  • Company
    • Purpose
      • Approach
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Our Story
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Leadership
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Careers
      • Careers Home
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Why Revvity
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Search Jobs
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • Investor Relations
      • Events
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      • Financials
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      • Stock Info
        Investor Day 2024

        Driving meaningful innovation that profoundly impacts science and human lives.

        Learn more

      Investor Day 2024

      Driving meaningful innovation that profoundly impacts science and human lives.

      Learn more

    • ESG
      • Environmental
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Social
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Governance
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    • News
      • Announcements
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Awards
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Media Alerts
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      • Press Coverage
        10% OFF sample prep instruments, accessories, and consumables!

        Terms and conditions apply.

        Learn more

      10% OFF sample prep instruments, accessories, and consumables!

      Terms and conditions apply.

      Learn more

    10% OFF sample prep instruments, accessories, and consumables!

    Terms and conditions apply.

    Learn more

  • Resources
    • Product Support
      • Application Support Knowledge base (ASK)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SDS Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • COA/TDS Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Manual/IFU Search
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SpectraViewer
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • RAD Calculator
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Resource Center
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Blog
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Events
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Customer Training
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Help Center
      • Order Support
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Contact Us
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Technical Support
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Instruments Support & Service
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • SDS Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • COA/TDS Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Manual/IFU Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Training Request
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Cell Line Terms & Conditions
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • FAQs
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Software Downloads
      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    • Knowledge Base
      • Application support knowledge base (ASK)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Sample homogenization applications and protocols
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Newborn screening disorders
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • TB testing services
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Investigator led studies
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      • Tuberculosis (TB)
        Tech documents, at your fingertips.

        Quickly find and download manuals, safety documents, certificates of analysis and more.

        Search now

      Tech documents, at your fingertips.

      Quickly find and download manuals, safety documents, certificates of analysis and more.

      Search now

    Tech documents, at your fingertips.

    Quickly find and download manuals, safety documents, certificates of analysis and more.

    Search now

  • Brands
      Revvity Brands
    • BioLegend

    • EUROIMMUN

    • Revvity Omics

    • Revvity Signals

    • Tulip Diagnostics

    • ViaCord

    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

    • View all Revvity brands
  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

    View our story

    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

Shop
Request a Quote
Contact Us
US
Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

australia.webp Australia
austria.webp Austria
belgium.webp Belgium
brazil.webp Brazil *
canada.webp Canada
china.webp China *
denmark.webp Denmark
finland.webp Finland
france.webp France
germany.webp Germany
hong-kong.webp Hong Kong *
india.webp India *
ireland.webp Ireland
italy.webp Italy
japan.webp Japan *
luxembourg.webp Luxembourg
mexico.webp Mexico *
netherlands.webp Netherlands
norway.webp Norway
philippines.webp Philippines *
republic of korea.webp Republic of Korea *
singapore.webp Singapore *
spain.webp Spain
sweden.webp Sweden
switzerland.webp Switzerland
thailand.webp Thailand *
uk.webp United Kingdom
usa.webp United States
Login/Register here
Revvity web shop online account

Get exclusive pricing on all online purchases.

Login to your Revvity.com account for your account's pricing, easy re-ordering from favorites & order history, priority order processing, and dynamic order tracking.

Login Register
Revvity Omics portal accounts

Initiate a new order or access test status and results for clinical genomics or newborn screening services.

View login options
Breadcrumb
...
  • Home
  • Blog
  • Assay Development Workflows
  • AlphaLISA technology: implementing a rapid, high-throughput screening method for immunomodulatory compounds.
img-implementing1920x640

Blog

Assay Development Workflows

May 23rd 2025

4 min read

AlphaLISA technology: implementing a rapid, high-throughput screening method for immunomodulatory compounds.

Help us improve your Revvity blog experience!

Feedback

The discovery of novel immunomodulatory compounds and effective vaccine adjuvants is a critical focus in immunology research. However, traditional methods for identifying such compounds often face limitations in throughput, scalability, and reproducibility. High-throughput screening (HTS) has transformed this field, serving as an essential tool for the rapid evaluation of thousands of compounds to assess cytotoxicity, immunological, and phenotypic effects. To address this challenge, Chew et al [1] has developed a HTS protocol utilizing human peripheral blood mononuclear cells (PBMCs) to model immune responses.

This innovative approach combines the sensitivity of AlphaLISA™ immunoassays with flow cytometry to measure key cytokine secretion from stimulated PBMCs, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-10 (IL-10), along with cell surface activation markers in response to compound exposure. The use of cryopreserved PBMCs in this study ensures reproducibility, while the integrated workflow offers a rapid and scalable approach to discovering new immunomodulators.

Four-step high-throughput workflow:
  • Step 1: Thawing cryopreserved PBMCs on Day 0
  • Step 2: Splitting the supernatant and cells on Day 3 to prepare for cytokine measurement using AlphaLISA and flow cytometry
  • Step 3: Cells are then fixated and stained on a microplate for analysis
  • Step 4: Quantitative readouts for cell surface marker expression via flow cytometry along with measurement using AlphaLISA technology to evaluate cytokine secretion levels (e.g., TNF-α, IFN-γ, IL-10) from the collected supernatants to determine the immunomodulatory potential of the compounds assessed.
What is AlphaLISA technology?

AlphaLISA (Amplified Luminescent Proximity Homogeneous Assay) is a fast, highly sensitive, homogeneous, no-wash assay platform performed in a microplate format that has been integrated into this screening protocol to measure immune responses accurately. The platform utilizes Alpha Donor and Acceptor beads, which, when brought into proximity by the binding of the target analyte, produce a luminescent signal upon excitation (Figure 1).
 

hts4


Figure 1. Schematic of AlphaLISA technology, a fast, highly sensitive, homogeneous, no-wash assay platform utilizing Donor and Acceptor beads for precise detection of molecules of interest. The workflow includes sample and bead addition, luminescence detection via a plate reader, and signal readout.

AlphaLISA’s cutting-edge technology offers several advantages:
  • High sensitivity and specificity: AlphaLISA provides a robust signal with minimal background interference, allowing for the precise quantification of low-abundance cytokines.
  • Homogeneous, no-wash assay format: The no-wash protocol simplifies the workflow, reducing assay time and minimizing potential errors associated with washing steps.
  • Compatibility with HTS: The technology is amenable to automation and miniaturization, making it suitable for high-throughput applications.

The study demonstrated that cryopreserved PBMCs stimulated in autologous plasma for 72 hours using small molecule libraries elicited distinct cytokine profiles for TNF-α, IFN-γ, and IL-10, which were precisely measured using AlphaLISA technology. The results obtained highlight the platform’s precision and utility in high-throughput detection of immune responses. Positive controls (R848 and ODN-2395) demonstrated significant cytokine induction compared to negative controls (DMSO), validating the assay’s sensitivity and reproducibility. Overall, these results support the use of AlphaLISA for high-through immune response detection and profiling experimental compounds. Key findings:

  1. AlphaLISA enables precise and sensitive cytokine profiling:

    The study demonstrated that cryopreserved PBMCs stimulated in autologous plasma for 72 hours using small molecule libraries elicited distinct cytokine profiles for TNF-α, IFN-γ, and IL-10, which were precisely measured using AlphaLISA technology. The results obtained highlight the platform’s precision and utility in high-throughput detection of immune responses. Positive controls (R848 and ODN-2395) demonstrated significant cytokine induction compared to negative controls (DMSO), validating the assay’s sensitivity and

  2. Comprehensive immune activation insights via flow cytometry:

    Complementary flow cytometry data enriched the cytokine findings by providing phenotypic insights into immune activation. Cell surface markers, including CD80, CD86, HLA-DR, and OX40, were analyzed to characterize activation states across various immune cell subsets. These markers indicated activation of specific immune cell subsets, including monocytes, B cells, and T cells. Compounds inducing heightened expression of these markers were flagged as potential immunomodulators. The dual-platform approach of integrating AlphaLISA and flow cytometry solidified the reliability and depth of the immunological profiling.

Conclusion:

This study showcases the power of AlphaLISA technology in a high-throughput setting for immunomodulator screening. By combining cytokine detection with flow cytometry, the authors developed a multiplexed platform capable of identifying potential therapeutic candidates efficiently. AlphaLISA’s advantages, including its no-wash format and scalability, underscore its potential for broad applications in immunological research and beyond.

Future directions for this study may include expanding the cytokine panel and integrating other cell types to explore a wider array of immune responses. Overall, this study reaffirms AlphaLISA’s role as a transformative tool in biomedical research.

AlphaLISA Kits Used in Chew et al.’s Study*:
  • TNF-a AlphaLISA Detection Kit, 5,000 Assay Points (Revvity, formerly PerkinElmer); Product Number: AL208: AlphaLISA High Performance Human TNFα Detection Kit, AL3157 AlphaLISA Human TNF-α High Performance Detection Kit, 100 Assay Points Discover
  • IFN-g AlphaLISA Detection Kit, 5,000 Assay Points (Revvity, formerly PerkinElmer); Product Number: AL217: AlphaLISA High Performance Human IFNγ Detection Kit, AL3153 AlphaLISA Human IFN-γ High Performance Detection Kit, 100 Assay Points Discover
  • IL-10 AlphaLISA Detection Kit, 5,000 Assay Points (Revvity, formerly PerkinElmer); Product Number: AL218: AlphaLISA Human IL-10 High Performance Detection Kit, AL3159 AlphaLISA Human IL-10 High Performance Detection Kit, 100 Assay Points Discover

*Note: All kits that were used in the study have been replaced with AlphaLISA High Performance kits.

Read the full protocol here.

Learn more about Revvity's AlphaLISA technology.
 

Dig deeper

References:
  1. Chew K, Lee B, Ozonoff A, Smith JA, Levy O, Dowling DJ, Van Haren S. A protocol for high-throughput screening for immunomodulatory compounds using human primary cells. STAR Protoc. 2023 Sep 15;4(3):102405. doi: 10.1016/j.xpro.2023.102405. Epub 2023 Jul 13. PMID: 37453068; PMCID: PMC10365952.

Help us improve your Revvity blog experience!

Feedback

Share this post:

  • Email
  • Facebook
  • Linkedin
  • Twitter

More Assay Development Workflows posts

Evaluating next-generation cytokines for cancer immunotherapy.
Read

Questions?
We’re here to help.

Contact us
Revvity Logo
  • Company
    • Purpose
    • Approach
    • Our Story
    • Leadership
    • Careers
    • News
    • Investor Relations
    • ESG
  • Connect
    • Contact Us
    • Offices & Authorized Distributors
    • Newsletter
    • BioLegend
    • EUROIMMUN
    • Revvity Signals
    • Tulip Diagnostics
    • ViaCord
  • Policies
    • All Policies
    • Privacy Policy
    • Terms & Conditions
    • Cookie Policy
    • Accessibility
  • Support
    • Order Support
    • Technical Support
    • Customer Care
    • Request Service Visit
    • Customer Training
    • Sales
    • Contact Us By Phone
    • FAQs
  • Facebook
    FB
  • Instagram
    IG
  • LinkedIn
    LI
  • Twitter
    X
  • Youtube
    YT

©2025 Revvity - All rights reserved

Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.