Celigo direct cell counting benefits
- Rapid scanning and analysis of multi-well plates; 384-well plate can be analyzed in <5 min
- Easy-to-use interface to image and identify a wide variety of cells in brightfield
- Determine growth characteristics of cells in situ, directly where they are grown
- Reports growth curves, cell counts, confluence, doubling time and doubling rate for each well
- Analyze cells growing in T-flasks (T-25 and T-75); T-75 flasks can be analyzed in ~15 min
Growth tracking of adherent cells during or post drug treatment
Determination of the growth characteristics of primary cells and cell lines requires live cell analysis methods and can be accurately analyzed in only a few minutes using the Celigo™ image cytometer. The Celigo provides a unique combination of brightfield imaging, image segmentation and analysis tools for researchers to monitor their cell cultures. Specifically, whole-well imaging and label-free brightfield cell counting allow the Celigo user to generate growth curves over time and monitor cell counts, doubling time and doubling rate, and measure confluence at the individual well level.
Label-free, non-destructive, no need to trypsinize adherent cells
The growth tracking application automatically integrates label-free cell counts of the same well/flask from different time points to provide a live cell analysis method to directly measure growth rates and doubling times, which is also a good overall assessment of cell health.
Two live cell analysis methods to produce a label-free proliferation curve
Method 1 – Cell segmentation
The first method is to use cell segmentation, in which each cell is identified by the image analysis software to produce cell counts. This method requires customization of the analysis parameters for each cell type.
Method 2 – Cell Confluence
The second method is to measure confluence of the cell colonies. This method is most universal and applicable to the majority of cell types without customization
Cell growth tracking using Celigo
Growth curves for CHO (DUXB11) cells grown in 96-well plates over 120 hours. Cells were imaged and counted using the Celigo Cell Counting application.
Streamlined cell culture maintenance using Celigo
Example of workflow and output for monitoring adherent cell cultures in 96-well plates. Cells can be imaged and counted in culture vessels without staining or harvesting. Celigo updates and returns growth curves automatically.
Cell Count
Cell growth curves are displayed in a 96-well plate
Confluence
For research use only. Not for use in diagnostic procedures.