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  • Home
  • Knowledge Base
  • CellCountingSub Section
  • CPE of PI3 Virus on Vero Cells
Cell Counting and Image Cytometry

CPE of PI3 Virus on Vero Cells

Section
Modern Virology Assays
Celigo Applications
Cell Counting Method Selection
Cell Counting and Image Cytometry FAQs
Cell-based Assays for Bioprocessing
Cell-based Assays for Gene Therapy Development
Cellometer Applications
Modern Virology Assays
Sub Section
Cytopathic Effects
Antibody Binding Inhibition
Antibody Neutralization
Cytopathic Effects
Viral Titre
Topic
CPE of PI3 Virus on Vero Cells
CPE of ECMV on Baby Hamster Kidney Cells
CPE of SV-5 Virus on CHO-K1 Cells
CPE of Zika Virus on HMC3 and JEG-3 Cells
CPE of Measles Virus on MRC-5 Cells
CPE of PI3 Virus on Vero Cells

Cytopathic effects of PI3 virus on Vero cells measured by confluence percentages

In this experiment, the goal is to measure the time-course CPE of PI3 virus on the Vero host cells in order to determine the earliest time point that CPE can be automatically detected.

The Vero host cells are seeded in 6-well microplates, incubated and allowed to adhere overnight.  The host cells are inoculated with PI3 virus as the positive sample and media for the negative control.  The 6-well microplates are scanned at 10% of the well that required less than 1 min/plate and analyzed using Celigo from day 0 to 12.  The Vero cell confluence percentages are measured directly in brightfield imaging to determine the CPE.

PI3 virus confluence

Time-course brightfield and analyzed images (Confluence) for the positive PI3 virus. The reduction in confluence % of Vero cells indicates cytopathic effects.

PI3 time course confluence

Time-course brightfield and analyzed images (Confluence) for the negative media control.

PI3 time course confluence plot

Time-course confluence percentage results, showing the CPE occurring on day 5.
Example CPE measurement performed using the Celigo image cytometer:


For research use only. Not for use in diagnostic procedures.

ON THIS PAGE
  • Cytopathic effects of PI3 virus on Vero cells measured by confluence percentages
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