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Opera Phenix Plus High-Content Screening System

Knowledge base

The Opera Phenix™ Plus high-content imaging system is a premier confocal solution for your most demanding high content applications. Drawing on over 20 years of experience, the Opera Phenix Plus is designed for high-throughput imaging assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca²⁺ flux.

For research use only. Not for use in diagnostic procedures.

Part #: HH14001000

Imaging Modality:

Brightfield, Confocal, Digital phase contrast, Fluorescence

Request more information Request a quote

Opera Phenix Plus High-Content Screening System

Opera Phenix Plus High-Content Screening System

Request a quote Request more information

Product variant

Brightfield, Confocal, Digital phase contrast, Fluorescence

Imaging Modality: Brightfield, Confocal, Digital phase contrast, Fluorescence

Part #: HH14001000

Request information

Opera Phenix Plus High-Content Screening system for high-throughput high-content assays

Unlock deeper insights with the Opera Phenix Plus high-content imaging system

Make meaningful discoveries faster with our advanced high-content imaging solutions.

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High content imaging provides a greater depth of information than other approaches, allowing you to explore cellular processes in detail.

Quantify in detail

Analyze phenotypic changes at scale, from single cells to entire populations. High-content imaging empowers detailed quantification.

Enhance 3D imaging

Water immersion objectives elevate 3D image quality, revealing intricate details within complex biological structures.

Boost throughput

Add more cameras to increase speed and efficiency. High-content imaging accelerates your research pipeline.

Video overview

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Key features

Multiple cameras

Increase imaging speed by using multiple cameras and simultaneous acquisition, especially for extensive stacks for 3D models.

Microlens-enhanced pinhole

Microlenses increase the excitation efficiency allowing for a bigger pinhole to pinhole distance thereby reducing out of focus light in confocal imaging.

Automated water-immersion objectives

Improve image quality and get better data by enhancing the signal and improving the z resolution while capturing more light.

Proprietary Synchrony™ optics

Combine a microlens-enhanced pinhole disk with dual-view confocal optics. This minimizes spectral crosstalk during simultaneous acquisition by separating fluorescence excitation and emission. Provide greater speed and higher sensitivity.

Machine learning

Easily create algorithms without being an image analysis expert using our PhenoLOGIC proprietary machine-learning technology.

Intelligent image acquisition

Image only the objects you are interested in, centered at high magnification, thereby reducing imaging time and data size.

Powerfully simple analysis

Harmony™ high-content imaging and analysis software can make you more productive faster with ready made templates and simple steps to a custom analysis.

3D analysis

Explore your cell models by visualizing them in a 3D- and an XYZ-viewer and quantify volumetric and other 3D related phenotypic readouts.

Applications

Micro Physiological Systems (MPS)

Deepen understanding of 3D cell models with clearer images that reveal critical features and processes.

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Functional genomic screening

Create genetic evidence with ease by phenotyping cellular response to thousands of gene manipulations in a single experiment.

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Cell painting

Simplify multiparametric screening with out of the box reagents and analysis to reduce complexity (and create more time for you to focus on the science).

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Drug sensitivity testing

Determine rational treatment selections regimens by rapidly characterizing ex vivo cellular function in response to drug treatment.

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Fast response assays

Capture rapid cellular events and dynamic processes with fast frame rates and on-board liquid dispensing.

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What our clients say about us

Delivering the sensitivity, resolution, and throughput we rely on

High-content, high-throughput microscopy is central to our discovery of condensate modulators (c-mods), enabling us to measure complex cellular phenotypes at scale with precision and speed. The Opera Phenix™ system is an integral component of our fully automated screening platform, delivering the sensitivity, resolution, and throughput we rely on to drive our discovery pipeline forward.

Diana Mitrea, PhD

Head of Scientific and Corporate Communications, Dewpoint Therapeutics

Opera Phenix Plus family: typical configurations

Opera Phenix Plus Single

The same sensitivity and resolution as the rest of the Phenix family, with the ability to upgrade later with additional cameras.

Opera Phenix Plus Simultaneous

Higher speed, dual-camera system for multi-color, simultaneous confocal image acquisition and fast multiplexing.

Opera Phenix Plus FRET

With its five lasers and four-camera setup, it supports CFP/YFP FRET applications to map protein-protein interactions.

Opera Phenix Plus Screener

The ultimate in throughput and performance, it delivers four cameras and four higher powered lasers - supporting screening of large libraries.

Configuration details

		Single	Simultaneous	FRET	Screener
System Options	Number of cameras	1	2	4	4
	Camera upgrade path	2 or 4 cameras	4 cameras	-	-
	Automated water immersion lenses	✔	✔	✔	✔
	Transmitted light	✔	✔	✔	✔
	Environmental control	✔	optional	✔	optional
	On-board liquid handling	optional	optional	optional	optional
	Robotics/automation compatible	✔	✔	✔	✔
	Emission filters	8	up to 16	up to 14	up to 14
Acquisition speed	2D/3D	+/+	++/++	+++/++++	+++/++++
Lasers	375/425 nm	-	-	✔	-
	405 nm	✔	✔	-	✔
	488 nm	✔	✔	✔	✔
	561 nm	✔	✔	✔	✔
	640 nm	✔	✔	✔	✔
Imaging modes	Synchrony optics for minimized simultaneous imaging cross talk	-	✔	✔	✔
	Multi-color fluorescence imaging	✔	✔	✔	✔
	Multi-color simultaneous confocal imaging	-	✔	✔	✔
	3D imaging	✔	✔	✔	✔
	Brightfield and digital phase contrast	✔	✔	✔	✔
	Fluorescence widefield imaging	✔	✔	✔	✔
	Ratiometric FRET*	+	+	++	+
	Fast frame rate imaging	✔	✔	✔	✔

*CFP/YFP or spectrally similar pairs

Image acquisition and analysis simplified with Harmony software

Intuitive workflow

Harmony™ software offers an intuitive user interface that guides you from image acquisition to analysis and evaluation.

Templates for quick set-up

Templates allow you to set up acquisition channels and parameters efficiently.

Ready-made solutions

Choose from pre-built solutions for common image analysis tasks, simplifying your workflow.

Customizable building blocks

Create, configure, and customize your own high-content analysis applications using image analysis building blocks.

Advanced features

Harmony™ includes advanced analysis capabilities, such as texture and STAR morphology analysis, providing detailed descriptions of cellular morphology and robust differentiation of phenotypes.

Data management

The software automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined keywords and annotations.

Image gallery

Opera Phenix Plus High-Content Screening system with liquid handling module and fast imaging frame rate for fast-response assays.

Composite image of HeLa cells (untreated) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS cells stained with PhenoVue 561 Mitochondrial Stain (Orange), PhenoVue Fluor 647 Live Cell Tubulin Stain (Red) and PhenoVue Hoechst 33342 (Blue). Imaged on Opera Phenix Plus.

C2C12 cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus.

Zebrafish 72hpf - Neutrophils. Lateral positioning on agarose. Imaged by PreciScan on Phenix Plus, 20xAir, Widefield : Brighfield / Ds Red (MIP). Image provided by Institut Pasteur Paris (PBI).

Composite images of HeLa cells (treated with tetrandine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

CaFlux Assay iPSC-dervied neurons (nexel) stained with Cal-520 on Opera Phenix Plus (20xW).

Composite images of HeLa cells (treated with berberine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS cells stained with PhenoVue Fluor 568 - Phalloidin, PhenoVue Fluor 555 - WGA, PhenoVue Fluor 488 - Concanavalin A, PhenoVue Fluor 641 Mitochondrial Stain, and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus, 63X Water Confocal.

U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)

Composite images of HeLa cells (treated with fembendazole) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "

Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.

Arabidopsis cells imaged on the Opera Phenix high-content screening system.

HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.

Opera Phenix Plus liquid handling module

Opera Phenix Plus system with liquid handling module

Cell painting assay imaged on the Opera Phenix Plus system

U2OS cell imaging on the Opera Phenix Plus system

Live cell staining on the Opera Phenix Plus system

Zebra fish imaging on the Opera Phenix Plus system

Cell painting assay imaged on the Opera Phenix Plus system

Calcium flux assay imaged on the Opera Phenix Plus system

Cell painting assay imaged on the Opera Phenix Plus system

U2OS cell imaging on the Opera Phenix Plus system

5-plex staining on the Opera Phenix Plus system

Cell painting assay imaged on the Opera Phenix Plus system

Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system

Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system

Arabidopsis cells imaged on the Opera Phenix high-content screening system

HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.

Opera Phenix Plus liquid handling module

Opera Phenix Plus High-Content Screening system with liquid handling module and fast imaging frame rate for fast-response assays.

Progress faster with our verified solutions

Microplates for high-content imaging

Utilize PhenoPlate™ 384-well microplates designed for optimal performance in high-content imaging applications. Employ CellCarrier Spheroid ULA plates for 3D cell models.

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img-cellular-imaging-reagents-highres512x288

PhenoVue cellular imaging reagents

Ready-to-use kits and reagents with straightforward protocols. Extensively tested to provide optimal formulations and long-term stability.

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Automation and workstations

Improve throughput, productivity, and reduce variability and reagent costs. Benefit from automated workflows using the explorer™ G3 workstations for cell painting, 3D cell culture, or phenotypic screening.

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Efficient data management

Export results automatically into the Image Artist™ platform that uses high performance computing and an industry standard object store to provide a scalable, multi-user solution for image analysis and management.

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Advanced analysis with Signals One

Perform screening data analysis and validation. Conduct QC analyses, calculate reliable normalization, multivariate hit stratification, dose-response curves, and drug response profiling.

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Phenologic.AI enhanced image analysis

The Phenologic.AI software module harnesses the power of pre-trained deep neural networks (DNNs) to provide an efficient and reliable method for identifying cells and cellular nuclei within fluorescent and brightfield images.

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Product information

Overview

Opera Phenix Plus high-content screening system for your most demanding high-content applications.

Modular design adapts to your changing application needs.
Enhanced speed using a dual - or four-camera configuration with simultaneous imaging.
Synchrony Optics™ combines a microlens-enhanced Nipkow spinning disk with a pinhole distance optimized for thick and 3D samples.
Dual-view excitation of neighboring spectral channels minimizes crosstalk.
Custom-designed high-NA water immersion objectives capture more photons and provide high-image resolution even in thick samples.
Fast imaging frame rate of up to 100 fps and optional pipettor module captures fast cellular responses.